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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 39, Iss. 28 — Oct. 1, 2000
  • pp: 5187–5193

Third-Harmonic Generation Microscopy in Highly Scattering Media

Carlo Mar Blanca and Caesar Saloma  »View Author Affiliations


Applied Optics, Vol. 39, Issue 28, pp. 5187-5193 (2000)
http://dx.doi.org/10.1364/AO.39.005187


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Abstract

The performance of third-harmonic generation (THG) microscopy in highly scattering media is analyzed with the Monte Carlo technique. The three-dimensional point-spread function (PSF) of the laser-scanning THG microscope with a pulsed excitation light source is derived for both isotropic and anisotropic scattering media and at different h/ds values, where h is the scattering depth as measured from the geometric focus of the objective lens and ds is the mean free path of the scattering medium. The generated THG signal is detected by a large-area photodetector. The PSF of the THG microscope is given by the third power of the normalized distribution of the excitation beam near the beam focus. The behavior of the temporal broadening of the excitation pulse on the generated THG signal is also analyzed as a function of h/ds. The relative advantages and disadvantages of the THG microscope relative to the two-photon fluorescence microscope are discussed thoroughly.

© 2000 Optical Society of America

OCIS Codes
(110.7050) Imaging systems : Turbid media
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy

Citation
Carlo Mar Blanca and Caesar Saloma, "Third-Harmonic Generation Microscopy in Highly Scattering Media," Appl. Opt. 39, 5187-5193 (2000)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-39-28-5187


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References

  1. J. A. Squier, M. Müller, G. J. Brakenhoff, and K. R. Wilson, “Third harmonic generation microscopy,” Opt. Exp. 3, 315–324 (1998).
  2. D. Yelin and Y. Silberberg, “Laser scanning third-harmonic-generation microscopy in biology,” Opt. Exp. 5, 169–175 (1999).
  3. Y. Barad, H. Eisenberg, M. Horowitz, and Y. Silberberg, “Nonlinear scanning laser microscopy by third harmonic generation,” Appl. Phys. Lett. 70, 922–924 (1997).
  4. D. Yelin, Y. Silberberg, Y. Barad, and J. Patel, “Phase-matched third-harmonic generation in a nematic liquid crystal cell,” Phys. Rev. Lett. 82, 3046–3049 (1999).
  5. M. Muller, J. Squier, and G. Brakenhoff, “3D microscopy of transparent objects using third-harmonic generation,” J. Microsc. 191, 266–274 (1998).
  6. J. A. Squier and M. Müller, “Third-harmonic generation imaging of laser induced breakdown in glass,” Appl. Opt. 38, 5789–5794 (1999).
  7. T. Tsang, “Optical third-harmonic generation at interfaces,” Phys. Rev. A 52, 4116–4125 (1995).
  8. C. Palmes-Saloma and C. Saloma, “Long depth imaging of specific gene expressions in wholemount mouse embryos with single-photon excitation confocal fluorescence microscope and FISH,” J. Structural Biology (to be published).
  9. C. Blanca and C. Saloma, “Monte-Carlo analysis of two-photon imaging through a scattering medium,” Appl. Opt. 37, 8092–8102 (1998).
  10. C. Blanca and C. Saloma, “Efficient analysis of temporal broadening of a pulsed focused Gaussian beam in scattering media,” Appl. Opt. 38, 5433–5437 (1999).
  11. R. Newton, Scattering Theory of Waves and Particles (McGraw-Hill, New York, 1966).
  12. A. Siegman, Lasers (University Science Books, Mill Valley, Calif., 1986).
  13. V. Daria, C. Blanca, O. Nakamura, S. Kawata, and C. Saloma, “Image contrast enhancement for two-photon fluorescence microscopy in turbid medium,” Appl. Opt. 37, 7960–7967 (1998).
  14. E. Wolf and M. Born, Principles of Optics, 6th ed. (Pergamon, New York, 1980), pp. 647–656.
  15. V. Daria, C. Palmes-Saloma, K. Fujita, C. Saloma, O. Nakamura, H. Kondoh, and S. Kawata, “Long depth imaging of turbid biological samples by two-photon microscopy,” in Proceedings of the Nineteenth Meeting of the Japan Society for Laser Microscopy (Japan Society for Laser Microscopy, Tokyo, 1997), pp. 28–32.
  16. W. Cheong, S. Prahl, and A. Welch, “A review of the optical properties of biological tissues,” IEEE J. Quantum Electron. 26, 2166–2185 (1990).
  17. C. Saloma and M. O. Cambaliza, “Single Gaussian beam interaction with a dielectric microsphere: radiation force, multiple internal reflections and caustic structures,” Appl. Opt. 34, 3522–3528 (1995).
  18. C. Saloma, C. Palmes-Saloma, and H. Kondoh, “Site-specific confocal fluorescence imaging of biological microstructures in turbid medium,” Phys. Med. Bio. 47, 1741–1759 (1998).

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