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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 40, Iss. 34 — Dec. 1, 2001
  • pp: 6281–6291

Optical Property Measurements of Turbid Media in a Small-Volume Cuvette with Frequency-Domain Photon Migration

Olivier Coquoz, Lars O. Svaasand, and Bruce J. Tromberg  »View Author Affiliations


Applied Optics, Vol. 40, Issue 34, pp. 6281-6291 (2001)
http://dx.doi.org/10.1364/AO.40.006281


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Abstract

A frequency-domain photon migration (FDPM) technique is developed for quantitative measurement of the absorption and reduced scattering coefficients of highly turbid samples in a small-volume (0.45-ml) reflective cuvette. We present both an analytical model for the FDPM cuvette and its experimental verification, using calibrated phantoms and suspensions of living cells. FDPM model fits to experimental data demonstrate that the reduced scattering (μ<sub><i>s</i></sub>′) and absorption (μ<sub><i>a</i></sub>) coefficients can be derived with accuracies of 5–10% and 10–15%, respectively. Changing the cuvette wall reflectivity alters the frequency-dependent behavior of photon density waves (PDWs). For highly reflective wall boundaries (<i>R</i><sub>eff</sub> ≥ 90–95%), PDW confinement leads to substantial enhancement in both amplitude and phase compared with identical samples in infinite media. Results from experiments on microsphere suspensions are compared with predictions from Mie theory to assess the potential of this method to interpret scattering properties in terms of scatterer size and density. Optical property measurements of biological cell suspensions are reported, and the possibility of optically monitoring cell physiology in a carefully controlled environment is demonstrated.

© 2001 Optical Society of America

OCIS Codes
(170.5270) Medical optics and biotechnology : Photon density waves
(170.5280) Medical optics and biotechnology : Photon migration
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(290.0290) Scattering : Scattering
(290.4020) Scattering : Mie theory
(290.7050) Scattering : Turbid media

Citation
Olivier Coquoz, Lars O. Svaasand, and Bruce J. Tromberg, "Optical Property Measurements of Turbid Media in a Small-Volume Cuvette with Frequency-Domain Photon Migration," Appl. Opt. 40, 6281-6291 (2001)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-40-34-6281

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