OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 41, Iss. 10 — Apr. 1, 2002
  • pp: 1852–1857

Resolution improvement in two-photon fluorescence microscopy with a single-mode fiber

Damian Bird and Min Gu  »View Author Affiliations


Applied Optics, Vol. 41, Issue 10, pp. 1852-1857 (2002)
http://dx.doi.org/10.1364/AO.41.001852


View Full Text Article

Enhanced HTML    Acrobat PDF (460 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

The dependence of spectral broadening of an ultrashort-pulsed laser beam on the fiber length and the illumination power is experimentally characterized in order to deliver the laser for two-photon fluorescence microscopy. It is found that not only the spectral width but also the spectral blue shift increases with the fiber length and illumination power, owing to the nonlinear response in the fiber. For an illumination power of 400 mW in a 3-m-long single-mode fiber, the spectral blue shift is as large as 15 nm. Such a spectral blue shift enhances the contribution from the short-wavelength components within the pulsed beam and leads to an improvement in resolution under two-photon excitation, whereas the efficiency of two-photon excitation is slightly reduced because of the temporal broadening of the pulsed beam. The experimental measurement of the axial response to a two-photon fluorescence polymer block confirms this feature.

© 2002 Optical Society of America

OCIS Codes
(060.2430) Fiber optics and optical communications : Fibers, single-mode
(060.4370) Fiber optics and optical communications : Nonlinear optics, fibers
(110.2350) Imaging systems : Fiber optics imaging
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(190.4180) Nonlinear optics : Multiphoton processes

History
Original Manuscript: May 25, 2001
Revised Manuscript: November 19, 2001
Published: April 1, 2002

Citation
Damian Bird and Min Gu, "Resolution improvement in two-photon fluorescence microscopy with a single-mode fiber," Appl. Opt. 41, 1852-1857 (2002)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-41-10-1852


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. W. Denk, J. H. Strickler, W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248, 73–75 (1990). [CrossRef] [PubMed]
  2. D. W. Piston, M. S. Kirby, H. Cheng, W. J. Lederer, “Two-photon-excitation fluorescence imaging of three-dimensional calcium-ion activity,” Appl. Opt. 33, 662–669 (1994). [CrossRef] [PubMed]
  3. P. T. C. So, H. Kim, I. E. Kochevar, “Two-photon deep tissue ex vivo imaging of mouse dermal and subcutaneous structures,” Opt. Express 3, 339–350 (1998), http://www.opticsexpress.org . [CrossRef] [PubMed]
  4. T. Dabbs, M. Glass, “Single-mode fibers used as confocal microscope pinholes,” Appl. Opt. 31, 705–706 (1992). [CrossRef] [PubMed]
  5. P. M. Delaney, M. R. Harris, R. G. King, “Fiber-optic laser scanning confocal microscope suitable for fluorescence imaging,” Appl. Opt. 33, 573–577 (1994). [CrossRef] [PubMed]
  6. M. Gu, C. J. R. Sheppard, X. Gan, “Image formation in a fiber-optical confocal scanning microscope,” J. Opt. Soc. Am. A 8, 1755–1761 (1991). [CrossRef]
  7. M. Gu, D. K. Bird, “Fibre-optical double-pass confocal microscopy,” Opt. Laser Technol. 30, 91–93 (1998). [CrossRef]
  8. A. F. Gmitro, D. Aziz, “Confocal microscopy through a fiber-optic imaging bundle,” Opt. Lett. 18, 565–567 (1993). [CrossRef] [PubMed]
  9. A. Lago, A. T. Obeidat, A. E. Kaplan, J. B. Khurigan, P. L. Shkolnikov, “Two-photon-induced fluorescence of biological markers based on optical fibers,” Opt. Lett. 20, 2054–2056 (1995). [CrossRef] [PubMed]
  10. G. P. Agrawal, Nonlinear Fiber Optics (Academic, San Diego, Calif., 1989).
  11. S. W. Hell, M. Booth, S. Wilms, C. M. Schnetter, A. K. Kirsch, D. J. Arndtjovin, T. M. Jovin, “Two-photon near- and far-field fluorescence microscopy with continuous-wave excitation,” Opt. Lett. 23, 1238–1240 (1998). [CrossRef]
  12. R. Wolleschensky, T. Feurer, R. Sauerbrey, U. Simon, “Characterisation and optimisation of a laser-scanning microscope in the femtosecond regime,” Appl. Phys. B 67, 87–94 (1998). [CrossRef]
  13. M. Gu, Principles of Three-Dimensional Imaging in Confocal Microscopes (World Scientific, Singapore, 1996).
  14. M. Gu, T. Tannous, C. J. R. Sheppard, “Three-dimensional confocal fluorescence imaging under ultrashort pulse illumination,” Opt. Commun. 117, 406–412 (1995). [CrossRef]
  15. D. Day, M. Gu, “Effects of refractive-index mismatch on three-dimensional optical data storage density in a two-photon bleaching polymer,” Appl. Opt. 37, 6299–6304 (1998). [CrossRef]
  16. M. Born, E. Wolf, Principles of Optics (Pergamon, New York, 1980).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited