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Applied Optics

Applied Optics


  • Vol. 41, Iss. 19 — Jul. 1, 2002
  • pp: 3999–4007

Detection limit enhancement of fluorescent heterogeneities in turbid media by dual-interfering excitation

Xavier Intes, Yu Chen, Xingde Li, and Britton Chance  »View Author Affiliations

Applied Optics, Vol. 41, Issue 19, pp. 3999-4007 (2002)

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We report on a quantitative comparison between the single-source and the dual-interfering-source configurations for the detection of fluorescent heterogeneities embedded in a piecewise highly scattering homogeneous fluorescent background. The study is based on simulations with analytical solutions of the frequency-domain fluorescent diffuse photon density waves and practical signal-to-noise ratio considerations. Results show that dual-interfering sources outperform single-source techniques for the detection of heterogeneities in terms of fluorophore concentration and lifetime contrast. To detect the same inhomogeneity, less concentration and lifetime contrast is required with dual-interfering sources.

© 2002 Optical Society of America

OCIS Codes
(110.5100) Imaging systems : Phased-array imaging systems
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.5270) Medical optics and biotechnology : Photon density waves
(170.5280) Medical optics and biotechnology : Photon migration
(260.2510) Physical optics : Fluorescence

Original Manuscript: October 6, 2001
Revised Manuscript: February 28, 2002
Published: July 1, 2002

Xavier Intes, Yu Chen, Xingde Li, and Britton Chance, "Detection limit enhancement of fluorescent heterogeneities in turbid media by dual-interfering excitation," Appl. Opt. 41, 3999-4007 (2002)

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