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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 41, Iss. 28 — Oct. 1, 2002
  • pp: 6006–6017

Direct Raman imaging techniques for study of the subcellular distribution of a drug

Jian Ling, Steven D. Weitman, Michael A. Miller, Rodney V. Moore, and Alan C. Bovik  »View Author Affiliations


Applied Optics, Vol. 41, Issue 28, pp. 6006-6017 (2002)
http://dx.doi.org/10.1364/AO.41.006006


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Abstract

Direct Raman imaging techniques are demonstrated to study the drug distribution in living cells. The advantage of Raman imaging is that no external markers are required, which simplifies the sample preparation and minimally disturbs the drug mechanism during imaging. The major challenge in Raman imaging is the weak Raman signal. In this study, we present a Raman image model to describe the degradation of Raman signals by imaging processes. Using this model, we demonstrate special-purpose image-processing algorithms to restore the Raman images. The processing techniques are then applied to visualize the anticancer agent paclitaxel in living MDA-435 breast cancer cells. Raman images were obtained from a cancer cell before, during, and after drug treatment. The paclitaxel distribution illustrated in these images is explained by means of the binding characteristics of the paclitaxel and its molecular target—the microtubules. This result demonstrates that direct Raman imaging is a promising tool to study the distribution of a drug in living cells.

© 2002 Optical Society of America

OCIS Codes
(100.0100) Image processing : Image processing
(110.0110) Imaging systems : Imaging systems
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(180.0180) Microscopy : Microscopy

History
Original Manuscript: March 1, 2002
Revised Manuscript: May 16, 2002
Published: October 1, 2002

Citation
Jian Ling, Steven D. Weitman, Michael A. Miller, Rodney V. Moore, and Alan C. Bovik, "Direct Raman imaging techniques for study of the subcellular distribution of a drug," Appl. Opt. 41, 6006-6017 (2002)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-41-28-6006

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