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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 41, Iss. 34 — Dec. 2, 2002
  • pp: 7275–7283

Nanosizing of fluorescent objects by spatially modulated illumination microscopy

Antonio Virgilio Failla, Udo Spoeri, Benno Albrecht, Alexander Kroll, and Christoph Cremer  »View Author Affiliations


Applied Optics, Vol. 41, Issue 34, pp. 7275-7283 (2002)
http://dx.doi.org/10.1364/AO.41.007275


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Abstract

A new approach to measuring the sizes of small fluorescent objects by use of spatially modulated illumination (SMI) far-field light microscopy is presented. This method is based on SMI measurements combined with a new SMI virtual microscopy (VIM) data analysis calibration algorithm. Here, experimental SMI measurements of fluorescent objects with known diameter (size) were made. From the SMI data obtained, the size was determined in an independent way by use of the SMI VIM algorithm. The results showed that with SMI microscopy in combination with SMI VIM calibration, subwavelength object size measurements as small as 40 nm are experimentally feasible with high accuracy.

© 2002 Optical Society of America

OCIS Codes
(100.0100) Image processing : Image processing
(110.0180) Imaging systems : Microscopy
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.3170) Microscopy : Interference microscopy
(180.6900) Microscopy : Three-dimensional microscopy

History
Original Manuscript: February 20, 2002
Revised Manuscript: August 13, 2002
Published: December 1, 2002

Citation
Antonio Virgilio Failla, Udo Spoeri, Benno Albrecht, Alexander Kroll, and Christoph Cremer, "Nanosizing of fluorescent objects by spatially modulated illumination microscopy," Appl. Opt. 41, 7275-7283 (2002)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-41-34-7275

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