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Effect of a trapping force on a photon-counting histogram

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Abstract

A theoretical study of the effect of a harmonic trapping force on the distribution of the fluorescence photon counts from Brownian particles is reported. The research of by Chen, et al. [Biophys. J. 77, 553 (1999)] is extended. Simulations of the photon-counting histograms indicate that the trapping force parameters can be estimated from changes in the shapes of the histograms, at least for microspheres.

© 2002 Optical Society of America

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