In low flow rates, red blood cells (RBCs) fasten together along their axis of symmetry and form a so-called rouleaux. The scattering of He-Ne laser light by a rouleau consisting of <i>n</i> (2 ≤ <i>n</i> ≤ 8) average-sized RBCs is investigated. The interaction problem is treated numerically by means of an advanced axisymmetric boundary element—fast Fourier transform methodology. The scattering problem of one RBC was solved first, and the results showed that the influence of the RBC’s membrane on the scattering patterns is negligible. Thus the rouleau is modeled as an axisymmetric, homogeneous, low-contrast dielectric cylinder, on the surface of which appears, owing to aggregated RBCs, a periodic roughness along the direction of symmetry. The direction of the incident laser light is considered to be perpendicular to the scatterer’s axis of symmetry. The differential scattering cross sections in both perpendicular and parallel scattering planes and for all the scattering angles are calculated and presented in detail.
© 2002 Optical Society of America
Stephanos V. Tsinopoulos, Euripides J. Sellountos, and Demosthenes Polyzos, "Light Scattering by Aggregated Red Blood Cells," Appl. Opt. 41, 1408-1417 (2002)