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Applied Optics

Applied Optics


  • Vol. 42, Iss. 16 — Jun. 1, 2003
  • pp: 3073–3080

In vivo quantitative three-dimensional localization of tumor labeled with exogenous specific fluorescence markers

Israel Gannot, Avital Garashi, Gallya Gannot, Victor Chernomordik, and Amir Gandjbakhche  »View Author Affiliations

Applied Optics, Vol. 42, Issue 16, pp. 3073-3080 (2003)

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We introduce a diffused optical detection system based on the administration of a fluorophore-antibody conjugate to diseased tissue. The conjugate interacts with the antigens expressed by the diseased tissue, resulting in fluorescent labeling of the antigen. By combining an optical detection system with a reconstruction algorithm developed on the basis of the random-walk model, we were able to determine the position of the fluorophore (and, thus, of the diseased cells) in the tissue. We present three-dimensional reconstructions of the location of a fluorophore (FITC-fluorescein isothiocyanate) in the tongues of mice. Measurements were performed with the fluorophore embedded at various simulated depths. The simulations were performed with agarose-based gel slabs applied to the tongue as tissuelike phantoms. Reconstructed fluorophore locations agree well with the actual values.

© 2003 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

Original Manuscript: September 17, 2002
Revised Manuscript: January 9, 2003
Published: June 1, 2003

Israel Gannot, Avital Garashi, Gallya Gannot, Victor Chernomordik, and Amir Gandjbakhche, "In vivo quantitative three-dimensional localization of tumor labeled with exogenous specific fluorescence markers," Appl. Opt. 42, 3073-3080 (2003)

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