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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 42, Iss. 19 — Jul. 1, 2003
  • pp: 3780–3784

Axial resolution limit of a fiber-optic fluorescence probe

J. Balaji, Kanchan Garai, Subhasis Chakrabarti, and Sudipta Maiti  »View Author Affiliations


Applied Optics, Vol. 42, Issue 19, pp. 3780-3784 (2003)
http://dx.doi.org/10.1364/AO.42.003780


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Abstract

We examine the limit of spatial resolution achievable when a single optical fiber is used for excitation and collection of fluorescence from a bulk specimen. We calculate the probability of detecting a fluorescent particle as a function of its position relative to the fiber face, using excitation wavelength λ, radius a, numerical aperture N.A., and the particle’s fluorescence and absorbance spectra. Treating Rhodamine B as a model fluorescent analyte and using appropriate fiber parameters, we show that the maximum axial resolution (defined as the axial distance in a homogenous solution within which 50% of the detected signal originates) achievable is ∼10 μm. We experimentally measured the axial resolution for a 500-μM aqueous solution of Rhodamine B with λ = 543 nm, a = 1.31 μm, and a N.A. of 0.16 and found good qualitative agreement with the calculation.

© 2003 Optical Society of America

OCIS Codes
(060.0060) Fiber optics and optical communications : Fiber optics and optical communications
(060.2370) Fiber optics and optical communications : Fiber optics sensors
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy

History
Original Manuscript: November 22, 2002
Revised Manuscript: April 14, 2003
Published: July 1, 2003

Citation
J. Balaji, Kanchan Garai, Subhasis Chakrabarti, and Sudipta Maiti, "Axial resolution limit of a fiber-optic fluorescence probe," Appl. Opt. 42, 3780-3784 (2003)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-42-19-3780

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