A laser-induced breakdown spectroscopy technique for analyzing biological matter for the detection of biological hazards is investigated. Eight species were considered in our experiment: six bacteria and two pollens in pellet form. The experimental setup is described, then a cumulative intensity ratio is proposed as a quantitative criterion because of its linearity and reproducibility. Time-resolved laser-induced breakdown spectroscopy (TRELIBS) exhibits a good ability to differentiate among all these species, whatever the culture medium, the species or the strain. Thus we expect that TRELIBS will be a good candidate for a sensor of hazards either on surfaces or in ambient air.
© 2003 Optical Society of America
(120.1880) Instrumentation, measurement, and metrology : Detection
(120.6200) Instrumentation, measurement, and metrology : Spectrometers and spectroscopic instrumentation
(140.3440) Lasers and laser optics : Laser-induced breakdown
(160.4890) Materials : Organic materials
(350.5400) Other areas of optics : Plasmas
Stéphane Morel, Nicolas Leone, Philippe Adam, and Jacques Amouroux, "Detection of Bacteria by Time-Resolved Laser-Induced Breakdown Spectroscopy," Appl. Opt. 42, 6184-6191 (2003)