We have determined the glucose concentration of whole blood from mid-infrared spectra without sample preparation or use of chemical reagents. We selected 1119–1022 cm<sup>−1</sup> as the optimal wavelength range for our measurement by making a first-loading vector analysis based on partial least-squares regression. We examined the influence of hemoglobin on samples by using different calibration and prediction sets. The accuracy of glucose prediction depended on the hemoglobin level in the calibration model; the sample set should represent the entire range of hemoglobin concentration. We obtained an accuracy of 5.9% in glucose prediction, and this value is well within a clinically acceptable range.
© 2003 Optical Society of America
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.1580) Medical optics and biotechnology : Chemometrics
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
Yoen-Joo Kim, Sangjoon Hahn, and Gilwon Yoon, "Determination of Glucose in Whole Blood Samples by Mid-Infrared Spectroscopy," Appl. Opt. 42, 745-749 (2003)