Information loss may occur frequently in the imaging of living tissues by using two-photon fluorescence microscopy due to the intensive deformation of the tissue. A landmark-based optical flow interpolation scheme is proposed for image reconstruction of living aorta walls in two-photon autofluorescence image sequences. Landmarks are extracted and evaluated by an active contour-based aorta model, and are aligned and reconstructed by use of a hierarchical algorithm. The accuracy of the calculation of optical flow is improved by applying landmark-based image warping. Experimental results show that the proposed scheme outperforms commonly used optical flow interpolation techniques for the reconstruction of intensively deforming tissues.
© 2003 Optical Society of America
(100.3020) Image processing : Image reconstruction-restoration
(110.0180) Imaging systems : Microscopy
(150.4620) Machine vision : Optical flow
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3880) Medical optics and biotechnology : Medical and biological imaging
Jue Wang, Liang Ji, and Hui Ma, "Reconstruction of Deforming Aortas in Two-Photon Autofluorescence Image Sequences," Appl. Opt. 42, 834-844 (2003)