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Applied Optics

Applied Optics


  • Vol. 42, Iss. 5 — Feb. 10, 2003
  • pp: 834–844

Reconstruction of deforming aortas in two-photon autofluorescence image sequences

Jue Wang, Liang Ji, and Hui Ma  »View Author Affiliations

Applied Optics, Vol. 42, Issue 5, pp. 834-844 (2003)

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Information loss may occur frequently in the imaging of living tissues by using two-photon fluorescence microscopy due to the intensive deformation of the tissue. A landmark-based optical flow interpolation scheme is proposed for image reconstruction of living aorta walls in two-photon autofluorescence image sequences. Landmarks are extracted and evaluated by an active contour-based aorta model, and are aligned and reconstructed by use of a hierarchical algorithm. The accuracy of the calculation of optical flow is improved by applying landmark-based image warping. Experimental results show that the proposed scheme outperforms commonly used optical flow interpolation techniques for the reconstruction of intensively deforming tissues.

© 2003 Optical Society of America

OCIS Codes
(100.3020) Image processing : Image reconstruction-restoration
(110.0180) Imaging systems : Microscopy
(150.4620) Machine vision : Optical flow
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3880) Medical optics and biotechnology : Medical and biological imaging

Original Manuscript: July 7, 2002
Revised Manuscript: October 21, 2002
Published: February 10, 2003

Jue Wang, Liang Ji, and Hui Ma, "Reconstruction of deforming aortas in two-photon autofluorescence image sequences," Appl. Opt. 42, 834-844 (2003)

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