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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 43, Iss. 23 — Aug. 10, 2004
  • pp: 4488–4496

Dualex: a new instrument for field measurements of epidermal ultraviolet absorbance by chlorophyll fluorescence

Yves Goulas, Zoran G. Cerovic, Aurélie Cartelat, and Ismaël Moya  »View Author Affiliations


Applied Optics, Vol. 43, Issue 23, pp. 4488-4496 (2004)
http://dx.doi.org/10.1364/AO.43.004488


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Abstract

Dualex (dual excitation) is a field-portable instrument, hereby described, for the assessment of polyphenolic compounds in leaves from the measurement of UV absorbance of the leaf epidermis by double excitation of chlorophyll fluorescence. The instrument takes advantage of a feedback loop that equalizes the fluorescence level induced by a reference red light to the UV-light-induced fluorescence level. This allows quick measurement from attached leaves even under field conditions. The use of light-emitting diodes and of a leaf-clip configuration makes Dualex a user-friendly instrument with potential applications in ecophysiological research, light climate analysis, agriculture, forestry, horticulture, pest management, selection of medicinal plants, and wherever accumulation of leaf polyphenolics is involved in plant responses to the environment.

© 2004 Optical Society of America

OCIS Codes
(120.6200) Instrumentation, measurement, and metrology : Spectrometers and spectroscopic instrumentation
(120.7000) Instrumentation, measurement, and metrology : Transmission
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(230.3670) Optical devices : Light-emitting diodes
(300.6540) Spectroscopy : Spectroscopy, ultraviolet

History
Original Manuscript: December 17, 2003
Revised Manuscript: May 10, 2004
Published: August 10, 2004

Citation
Yves Goulas, Zoran G. Cerovic, Aurélie Cartelat, and Ismaël Moya, "Dualex: a new instrument for field measurements of epidermal ultraviolet absorbance by chlorophyll fluorescence," Appl. Opt. 43, 4488-4496 (2004)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-43-23-4488

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