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Applied Optics

Applied Optics


  • Vol. 43, Iss. 23 — Aug. 10, 2004
  • pp: 4488–4496

Dualex: a new instrument for field measurements of epidermal ultraviolet absorbance by chlorophyll fluorescence

Yves Goulas, Zoran G. Cerovic, Aurélie Cartelat, and Ismaël Moya  »View Author Affiliations

Applied Optics, Vol. 43, Issue 23, pp. 4488-4496 (2004)

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Dualex (dual excitation) is a field-portable instrument, hereby described, for the assessment of polyphenolic compounds in leaves from the measurement of UV absorbance of the leaf epidermis by double excitation of chlorophyll fluorescence. The instrument takes advantage of a feedback loop that equalizes the fluorescence level induced by a reference red light to the UV-light-induced fluorescence level. This allows quick measurement from attached leaves even under field conditions. The use of light-emitting diodes and of a leaf-clip configuration makes Dualex a user-friendly instrument with potential applications in ecophysiological research, light climate analysis, agriculture, forestry, horticulture, pest management, selection of medicinal plants, and wherever accumulation of leaf polyphenolics is involved in plant responses to the environment.

© 2004 Optical Society of America

OCIS Codes
(120.6200) Instrumentation, measurement, and metrology : Spectrometers and spectroscopic instrumentation
(120.7000) Instrumentation, measurement, and metrology : Transmission
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(230.3670) Optical devices : Light-emitting diodes
(300.6540) Spectroscopy : Spectroscopy, ultraviolet

Original Manuscript: December 17, 2003
Revised Manuscript: May 10, 2004
Published: August 10, 2004

Yves Goulas, Zoran G. Cerovic, Aurélie Cartelat, and Ismaël Moya, "Dualex: a new instrument for field measurements of epidermal ultraviolet absorbance by chlorophyll fluorescence," Appl. Opt. 43, 4488-4496 (2004)

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  1. P. G. Waterman, S. Mole, Analysis of Phenolic Plant Metabolites, Methods in Ecology (Blackwell Scientific, Oxford, UK, 1994), p. 238.
  2. M. M. Caldwell, “Solar UV irradiation and the growth and development of higher plants,” in Photophysiology, A. C. Giese, ed. (Academic, New York, 1971), pp. 131–171.
  3. M. M. Caldwell, R. Robberecht, S. D. Flint, “Internal filters: prospects for UV-acclimation in higher plants,” Physiol. Plant. 58, 445–450 (1983). [CrossRef]
  4. P. D. Coley, J. P. Bryant, S. Chapin, “Resource availability and plant antiherbivore defense,” Science 230, 895–899 (1985). [CrossRef] [PubMed]
  5. K. Hahlbrock, D. Scheel, “Physiology and molecular biology of phenylpropanoid metabolism,” Annu. Rev. Plant Physiol. Plant Mol. Biol. 40, 347–369 (1989). [CrossRef]
  6. Z. G. Cerovic, A. Ounis, A. Cartelat, G. Latouche, Y. Goulas, S. Meyer, I. Moya, “The use of chlorophyll fluorescence excitation spectra for the non-destructive in situ assessment of UV-absorbing compounds in leaves,” Plant Cell Environ. 25, 1663–1676 (2002). [CrossRef]
  7. M. N. Merzlyak, A. E. Solovchenko, A. A. Gitelson, “Reflectance spectral features and non-destructive estimation of chlorophyll, carotenoid and anthocyanin content in apple fruit,” Postharvest Biol. Technol. 27, 197–211 (2003). [CrossRef]
  8. T. A. Day, B. W. Howells, W. J. Rice, “Ultraviolet absorption and epidermal-transmittance spectra in foliage,” Physiol. Plant. 92, 207–218 (1994). [CrossRef]
  9. J. F. Bornman, T. C. Vogelmann, “Penetration of blue and UV radiation measured by fiber optics in spruce and fir needles,” Physiol. Plant. 72, 699–705 (1988). [CrossRef]
  10. W. Bilger, M. Veit, L. Schreiber, U. Schreiber, “Measurement of leaf epidermal transmittance of UV radiation by chlorophyll fluorescence,” Physiol Plant 101, 754–763 (1997). [CrossRef]
  11. W. Bilger, T. Johnsen, U. Schreiber, “UV-excited chlorophyll fluorescence as a tool for the assessment of UV-protection by the epidermis of plants,” J. Exp. Bot. 52, 2007–2017 (2001). [CrossRef] [PubMed]
  12. P. Burchard, W. Bilger, G. Weissenbock, “Contribution of hydroxycinnamates and flavonoids to epidermal shielding of UV-A and UV-B radiation in developing rye primary leaves as assessed by ultraviolet-induced chlorophyll fluorescence measurements,” Plant Cell Environ. 23, 1373–1380 (2000). [CrossRef]
  13. G. Krause, E. Weis, “Chlorophyll fluorescence and photosynthesis: the basics,” Annu. Rev. Plant Physiol. Plant Mol. Biol. 42, 313–349 (1991). [CrossRef]
  14. P. W. Barnes, P. S. Searles, C. L. Ballare, R. J. Ryel, M. M. Caldwell, “Non-invasive measurements of leaf epidermal transmittance of UV radiation using chlorophyll fluorescence: field and laboratory studies,” Physiol. Plant. 109, 274–283 (2000). [CrossRef]
  15. H. W. Trissl, “Determination of the quenching efficiency of the oxidized primary donor of Photosystem I, P700+: implications for the trapping mechanism,” Photosynth. Res. 54, 237–240 (1997). [CrossRef]
  16. Z. G. Cerovic, Y. Goulas, I. Moya, “Device for measuring light absorption characteristics of a biological tissue sample,” Patent WO03029791 (10April2003).
  17. G. Samson, O. Prasil, B. Yaakoubd, “Photochemical and thermal phases of chlorophyll a fluorescence,” Photosynthetica 37, 163–182 (1999). [CrossRef]
  18. Z. G. Cerovic, G. Samson, F. Morales, N. Tremblay, I. Moya, “Ultraviolet-induced fluorescence for plant monitoring: present state and prospects,” Agron. Agric. Environ. 19, 543–578 (1999). [CrossRef]
  19. A. Cartelat, Z. Cerovic, Y. Goulas, S. Meyer, C. Lelarge, J.-L. Prioul, A. Barbottin, M.-H. Jeuffroy, P. Gate, G. Agati, I. Moya, “Optically assessed content of leaf polyphenolics and chlorophyll of leaves as indicators of nitrogen deficiency in wheat (Triticum aestivum L.),” Field Crops Res. (to be published).
  20. A. Ounis, Z. G. Cerovic, J. M. Briantais, I. Moya, “Dual-excitation FLIDAR for the estimation of epidermal UV absorption in leaves and canopies,” Remote Sens. Environ. 76, 33–48 (2001). [CrossRef]
  21. D. S. Ellsworth, P. B. Reich, “Leaf mass per area, nitrogen content and photosynthetic carbon gain in Acer saccharum seedlings in contrasting forest light environments,” Funct. Ecol. 6, 423–435 (1992). [CrossRef]
  22. J. Amesz, L. N. M. Duysens, D. C. Brandt, “Methods for measuring and correcting the absorption spectrum of scattering suspensions,” J. Theor. Biol. 1, 59–74 (1961). [CrossRef] [PubMed]
  23. D. A. Herms, W. J. Mattson, “The dilemma of plants: to grow or to defend,” Q. Rev. Biol. 67, 283–335 (1992). [CrossRef]
  24. A. Cartelat, Y. Goulas, C. Lelarge, A. Barbottin, M.-H. Jeuffroy, Z. G. Cerovic, “A new optical signature of nitrogen deficiency in wheat useful for decision support in precision agriculture,” in the Fourth European Conference on Precision Agriculture, A. Werner, A. Jarfe, eds. (WageningenAcademic, Berlin, 2003), pp. 377–379.

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