Experimental and theoretical investigation of fluorescence photobleaching and recovery in human breast tissue and tissue phantoms
Applied Optics, Vol. 43, Issue 5, pp. 1044-1052 (2004)
http://dx.doi.org/10.1364/AO.43.001044
Acrobat PDF (231 KB)
Abstract
Photobleaching and recovery of 488-nm excited fluorescence from resected human breast tissue samples have been studied. Profiles of photobleaching decay were seen to be faster in cancerous tissue than in those of the normal tissue. The reverse behavior was observed in profiles of recovery after photobleaching. A theoretical model based on one-dimensional diffusion theory has been developed to provide insight into the phenomena of fluorescence during photobleaching and recovery in a multiply scattering medium such as tissue. To understand photobleaching and recovery with the help of this theoretical model, we carried out experiments with model media that were prepared with authentic fluorophores, scatterers, and absorbers. The results of these studies suggest that the fluorescence photobleaching profiles are affected more by the absorption than by the scattering properties of a turbid medium such as tissue. In contrast, the scattering properties of the medium are found to affect the fluorescence recovery profiles to a greater extent. These observations could be related to the observed difference in fluorescence photobleaching and recovery profiles of normal and cancerous breast tissues.
© 2004 Optical Society of America
OCIS Codes
(160.4760) Materials : Optical properties
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(260.2510) Physical optics : Fluorescence
(290.1990) Scattering : Diffusion
Citation
Sharad Gupta, Bhawna Bhawna, Pallab Goswami, Asha Agarwal, and Asima Pradhan, "Experimental and theoretical investigation of fluorescence photobleaching and recovery in human breast tissue and tissue phantoms," Appl. Opt. 43, 1044-1052 (2004)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-43-5-1044
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Diffusion coefficient in photon diffusion theory (OL)
- Spatially resolved backscattering: implementation of extrapolation boundary condition and exponential source (JOSAA)
- Photon diffusion coefficient in an absorbing medium (JOSAA)
- Determination of optical parameters of human breast tissue from spatially resolved fluorescence: a diffusion theory model (AO)
- Simultaneous extraction of optical transport parameters and intrinsic fluorescence of tissue mimicking model media using a spatially resolved fluorescence technique (AO)
Related Conference Papers
- Bridging the gap between Yb3+ emission and Nd3+ emission in apatite crystals
- Experimental Assessment of Analytical Models for Estimating Tumor Optical Properties in Laser Pulse Mammography
- Studies of Absorption and Scattering of Light on a Model Coral
- Scattering Characterization of TiO2/Polyurethane Phantom Using Frequency-Domain Optical Imaging
- Why Acquiring Excitation Data Improves the Quality of Reconstructed Fluorescence Images for Highly Heterogeneous Diffusive Media
« Previous Article | Next Article »
OSA is a member of 