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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 44, Iss. 10 — Apr. 1, 2005
  • pp: 1792–1797

In vivo fiber-optic confocal reflectance microscope with an injection-molded plastic miniature objective lens

Kristen Carlson, Matthew Chidley, Kung-Bin Sung, Michael Descour, Ann Gillenwater, Michele Follen, and Rebecca Richards-Kortum  »View Author Affiliations


Applied Optics, Vol. 44, Issue 10, pp. 1792-1797 (2005)
http://dx.doi.org/10.1364/AO.44.001792


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Abstract

For in vivo optical diagnostic technologies to be distributed to the developed and developing worlds, optical imaging systems must be constructed of inexpensive components. We present a fiber-optic confocal reflectance microscope with a cost-effective injection-molded plastic miniature objective lens for in vivo imaging of human tissues in near real time. The measured lateral resolution is less than 2.2 µm, and the measured axial resolution is 10 µm. Confocal images of ex vivo cervical tissue biopsies and in vivo human lip taken at 15 frames/s demonstrate the microscope’s capability of imaging cell morphology and tissue architecture.

© 2005 Optical Society of America

OCIS Codes
(170.1610) Medical optics and biotechnology : Clinical applications
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4440) Medical optics and biotechnology : ObGyn
(170.4730) Medical optics and biotechnology : Optical pathology

History
Original Manuscript: July 20, 2004
Revised Manuscript: November 23, 2004
Manuscript Accepted: November 23, 2004
Published: April 1, 2005

Citation
Kristen Carlson, Matthew Chidley, Kung-Bin Sung, Michael Descour, Ann Gillenwater, Michele Follen, and Rebecca Richards-Kortum, "In vivo fiber-optic confocal reflectance microscope with an injection-molded plastic miniature objective lens," Appl. Opt. 44, 1792-1797 (2005)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-44-10-1792


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