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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 44, Iss. 10 — Apr. 1, 2005
  • pp: 1823–1830

Determination of optimal exposure time for imaging of blood flow changes with laser speckle contrast imaging

Shuai Yuan, Anna Devor, David A. Boas, and Andrew K. Dunn  »View Author Affiliations


Applied Optics, Vol. 44, Issue 10, pp. 1823-1830 (2005)
http://dx.doi.org/10.1364/AO.44.001823


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Abstract

Laser speckle contrast imaging is becoming an established method for full-field imaging of cerebral blood flow dynamics in animal models. The sensitivity and noise in the measurement of blood flow changes depend on the camera exposure time. The relation among sensitivity, noise, and camera exposure time was investigated experimentally by imaging the speckle contrast changes in the brain after electrical forepaw stimulation in rats. The sensitivity to relative changes in speckle contrast was found to increase at longer exposure times and to reach a plateau for exposure times greater than approximately 2 ms. However, the speckle contrast noise also increases with exposure time and thus the contrast-to-noise ratio was found to peak at an exposure time of approximately 5 ms. Our results suggests that ~5 ms is an optimal exposure time for imaging of stimulus-induced changes in cerebral blood flow in rodents.

© 2005 Optical Society of America

OCIS Codes
(120.6150) Instrumentation, measurement, and metrology : Speckle imaging
(170.1650) Medical optics and biotechnology : Coherence imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging

History
Original Manuscript: August 10, 2004
Revised Manuscript: November 16, 2004
Manuscript Accepted: December 5, 2004
Published: April 1, 2005

Citation
Shuai Yuan, Anna Devor, David A. Boas, and Andrew K. Dunn, "Determination of optimal exposure time for imaging of blood flow changes with laser speckle contrast imaging," Appl. Opt. 44, 1823-1830 (2005)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-44-10-1823


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