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Applied Optics

Applied Optics


  • Vol. 44, Iss. 11 — Apr. 10, 2005
  • pp: 2154–2161

Improvement of transcutaneous fluorescent images with a depth-dependent point-spread function

Koichi Shimizu, Koji Tochio, and Yuji Kato  »View Author Affiliations

Applied Optics, Vol. 44, Issue 11, pp. 2154-2161 (2005)

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The point-spread function (PSF) for transcutaneous fluorescent imaging was obtained as an analytical solution in a closed form. It is applicable to cases in which the optical property of the image-blurring turbid medium is considered to be fairly homogeneous. We proposed a technique to improve a transcutaneous image by using depth-dependent PSF. Contrast of the fluorescent image was improved for depths of 1–15 mm in a scattering medium (μs = 1/mm). The visible depth was more than doubled with this technique. An experiment with a rat demonstrated considerable improvement of a transcutaneous image of the cerebral vein at a specified depth. The spread image of the heart was reduced to the correct size by use of the PSF with the actual depth of the heart.

© 2005 Optical Society of America

OCIS Codes
(100.1830) Image processing : Deconvolution
(100.3020) Image processing : Image reconstruction-restoration
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.7050) Medical optics and biotechnology : Turbid media

Original Manuscript: July 21, 2004
Revised Manuscript: December 12, 2004
Manuscript Accepted: January 11, 2005
Published: April 10, 2005

Koichi Shimizu, Koji Tochio, and Yuji Kato, "Improvement of transcutaneous fluorescent images with a depth-dependent point-spread function," Appl. Opt. 44, 2154-2161 (2005)

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