Selective fluorescence excitation of specific molecular species is demonstrated by using coherent control of two-photon excitation with supercontinuum pulses generated with a microstructure fiber. Pulse shaping prior to pulse propagation through the fiber is controlled by a self-learning optimization loop so that the highest fluorescence signal contrast between two fluorescent proteins is obtainable. The self-learning optimization loop successfully controls both the optical nonlinarity of the microstructure fiber and the two-photon excitation of the fluorescent proteins.
© 2007 Optical Society of America
Original Manuscript: October 24, 2006
Revised Manuscript: January 9, 2007
Manuscript Accepted: January 11, 2007
Published: May 1, 2007
Vol. 2, Iss. 6 Virtual Journal for Biomedical Optics
Junji Tada, Taiki Kono, Akira Suda, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa, and Fumihiko Kannari, "Adaptively controlled supercontinuum pulse from a microstructure fiber for two-photon excited fluorescence microscopy," Appl. Opt. 46, 3023-3030 (2007)