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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 46, Iss. 22 — Aug. 1, 2007
  • pp: 5110–5118

Estimating the cell density and invasive radius of three-dimensional glioblastoma tumor spheroids grown in vitro

Andrew M. Stein, Michal O. Nowicki, Tim Demuth, Michael E. Berens, Sean E. Lawler, E. Antonio Chiocca, and Leonard M. Sander  »View Author Affiliations

Applied Optics, Vol. 46, Issue 22, pp. 5110-5118 (2007)

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To gain insight into brain tumor invasion, experiments are conducted on multicellular tumor spheroids grown in collagen gel. Typically, a radius of invasion is reported, which is obtained by human measurement. We present a simple, heuristic algorithm for automated invasive radii estimation (AIRE) that uses local fluctuations of the image intensity. We then derive an analytical expression relating the image graininess to the cell density for a model imaging system. The result agrees with the experiment up to a multiplicative constant and thus describes a novel method for estimating the cell density from bright-field images.

© 2007 Optical Society of America

OCIS Codes
(100.2000) Image processing : Digital image processing
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Imaging Systems

Original Manuscript: February 15, 2007
Revised Manuscript: May 7, 2007
Manuscript Accepted: May 19, 2007
Published: July 9, 2007

Virtual Issues
Vol. 2, Iss. 9 Virtual Journal for Biomedical Optics

Andrew M. Stein, Michal O. Nowicki, Tim Demuth, Michael E. Berens, Sean E. Lawler, E. Antonio Chiocca, and Leonard M. Sander, "Estimating the cell density and invasive radius of three-dimensional glioblastoma tumor spheroids grown in vitro," Appl. Opt. 46, 5110-5118 (2007)

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