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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 46, Iss. 22 — Aug. 1, 2007
  • pp: 5384–5395

Adaptive interferometry of protein on a BioCD

Leilei Peng, Manoj M. Varma, Wonryeon Cho, Fred E. Regnier, and David D. Nolte  »View Author Affiliations

Applied Optics, Vol. 46, Issue 22, pp. 5384-5395 (2007)

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Adaptive spinning-disk interferometry is capable of measuring surface profiles of a thin biolayer with subnanometer longitudinal resolution. High-speed phase modulation in the signal beam arises from the moving surface height profile on the spinning disk and is detected as a homodyne signal via dynamic two-wave mixing. A photorefractive quantum-well device performs as an adaptive mixer that compensates disk wobble and vibration while it phase-locks the signal and reference waves in the phase quadrature condition ( π / 2 relative phase between the signal and local oscillator). We performed biosensing of immobilized monolayers of antibodies on the disk in both transmission and reflection detection modes. Single- and dual-analyte adaptive spinning-disk immunoassays were demonstrated with good specificity and without observable cross-reactivity. Reflection-mode detection enhances the biosensing sensitivity to one-twentieth of a protein monolayer, creates a topographic map of the protein layer, and can differentiate monolayers of different species by their effective optical thicknesses.

© 2007 Optical Society of America

OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(160.5320) Materials : Photorefractive materials
(170.3890) Medical optics and biotechnology : Medical optics instrumentation

ToC Category:

Original Manuscript: February 26, 2007
Manuscript Accepted: April 18, 2007
Published: July 23, 2007

Virtual Issues
Vol. 2, Iss. 9 Virtual Journal for Biomedical Optics

Leilei Peng, Manoj M. Varma, Wonryeon Cho, Fred E. Regnier, and David D. Nolte, "Adaptive interferometry of protein on a BioCD," Appl. Opt. 46, 5384-5395 (2007)

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  1. M. M. Varma, D. D. Nolte, H. D. Inerowicz, and F. E. Regnier, "Spinning-disk self-referencing inteferometry of antigen-antibody recognition," Opt. Lett. 29, 950-952 (2004). [CrossRef] [PubMed]
  2. L. Peng, M. M. Varma, F. E. Regnier, and D. D. Nolte, "Adaptive optical biocompact disk for molecular recognition," Appl. Phys. Lett. 86, 183902 (2005). [CrossRef]
  3. A. Blouin and J.-P. Monchalin, "Detection of ultrasonic motion of a scattering surface by two-wave mixing in a photorefractive GaAs crystal," Appl. Phys. Lett. 65, 932-934 (1994). [CrossRef]
  4. F. M. Davidson and L. Boutsikaris, "Homodyne detection using photorefractive materials as beamsplitters," Opt. Eng. 29(4), 369-377 (1990). [CrossRef]
  5. J. Khoury, V. Ryan, C. Woods, and M. Cronin-Golomb, "Photorefractive optical lock-in detector," Opt. Lett. 16, 1442-1444 (1991). [CrossRef] [PubMed]
  6. L. A. de Montmorillon, I. Biaggio, P. Delaye, J. C. Launay, and G. Roosen, "Eye-safe large field of view homodyne detection using a photorefractive CdTe:V crystal," Opt. Commun. 129, 293-300 (1996). [CrossRef]
  7. I. Rossomakhin and S. I. Stepanov, "Linear adaptive interferometers via diffusion recording in cubic photorefractive crystals," Opt. Commun. 86, 199-204 (1991). [CrossRef]
  8. R. K. Ing and J.-P. Monchalin, "Broadband optical detection of ultrasound by two-wave mixing in a photorefractive crystal," Appl. Phys. Lett. 59, 3233-3235 (1991). [CrossRef]
  9. I. Lahiri, L. J. Pyrak-Nolte, D. D. Nolte, M. R. Melloch, R. A. Kruger, G. D. Bacher, and M. B. Klein, "Laser-based ultrasound detection using photorefractive quantum wells," Appl. Phys. Lett. 73, 1041-1043 (1998). [CrossRef]
  10. B. F. Pouet, R. K. Ing, and S. Krishnaswamy, "Heterodyne interferometer with two-wave mixing in photorefractive crystals for ultrasound detection on rough surfaces," Appl. Phys. Lett. 69, 3782 (1996). [CrossRef]
  11. P. Yu, L. Peng, D. D. Nolte, and M. R. Melloch, "Ultrasound detection through turbid media," Opt. Lett. 28, 819-891 (2003). [CrossRef] [PubMed]
  12. S. Stepanov, V. Petrov, P. Rodriguez, and R. Lopez, "Directional detection of laser-generated ultrasound with an adaptive two-wave mixing photorefractive configuration," Opt. Commun. 187, 249-255 (2001). [CrossRef]
  13. P. Delaye, A. Blouin, D. Drolet, L.-A. Montmorillon, G. Roosen, and J.-P. Monchalin, "Detection of ultrasonic motion of a scattering surface by photorefractive InP:Fe under an applied dc field," J. Opt. Soc. Am. B 14, 1723-1734 (1997). [CrossRef]
  14. Q. Wang, R. M. Brubaker, D. D. Nolte, and M. R. Melloch, "Photorefractive quantum wells: transverse Franz-Keldysh geometry," J. Opt. Soc. Am. B 9, 1626-1641 (1992). [CrossRef]
  15. S. Balasubramanian, I. Lahiri, Y. Ding, M. R. Melloch, and D. D. Nolte, "Two-wave mixing dynamics and nonlinear hot-electron transport in transverse-geometry photorefractive quantum wells studied by moving gratings," Appl. Phys. B 68, 863-869 (1999). [CrossRef]
  16. R. M. Brubaker, Q. N. Wang, and D. D. Nolte, "Nonlocal photorefractive screening from hot electron velocity saturation on semiconductors," Phys. Rev. Lett. 77, 4249-4252 (1996). [CrossRef] [PubMed]
  17. D. D. Nolte, T. Cubel, L. J. Pyrak-Nolte, and M. R. Melloch, "Adaptive beam combining and interferometry using photorefractive quantum wells," J. Opt. Soc. Am. B 18, 195-205 (2001). [CrossRef]
  18. B. J. Berne and R. Pecora, Dynamic Light Scattering: With Applications to Chemistry, Biology, and Physics (Dover, 2000).
  19. M.-L. Theye, "Investigation of the optical properties of Au by means of thin semitransparent films," Phys. Rev. B 2(8), 3060-3078 (1970). [CrossRef]
  20. J. Duchet, J. F. Gérard, J. P. Chapel, and B. Chabert, "Grafting of alkylchlorosilanes ontosilica from solution for adhesion enhancement," J. Adhes. Sci. Technol. 14(5), 691-718 (2000). [CrossRef]
  21. W. Cho, "A new biocompatible coating for bioanalytical devices based on PSI (polysuccinimide)," Ph.D. dissertation (Purdue University, 2006).
  22. E. Delamarche, A. Bernard, H. Schmid, B. Michel, and H. Biebuyck, "Patterned diversity of immunoglobulins to surfaces using microfluidic networks," Science 276, 779-781 (1997). [CrossRef] [PubMed]
  23. A. Bernard, J. P. Renault, B. Michel, H. R. Bosshard, and E. Delamarche, "Microcontact printing of proteins," Adv. Mater. 12, 1067-1070 (2000). [CrossRef]

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