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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 46, Iss. 29 — Oct. 10, 2007
  • pp: 7132–7140

Toward instrument-independent quantitative measurement of fluorescence intensity in fiber-optic spectrometer systems

Jianhua Zhao, Harvey Lui, David I. McLean, and Haishan Zeng  »View Author Affiliations

Applied Optics, Vol. 46, Issue 29, pp. 7132-7140 (2007)

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Fluorescence has been widely used in biological research and clinical diagnosis. One challenge facing the rapid growth of fluorescence application is the inability to make comparable fluorescence intensity measurements during a long period of clinical study and across laboratories. We propose a method to implement system-independent fluorescence intensity calibration in fiber-optic fluorescence spectrometer systems. This method is based on a National Institute for Standards and Technology traceable standard light source for system spectral response calibration, and a fluorescence reference standard for fluorescence intensity calibration. Human skin in vivo and a fluorescence phantom made of fluorescent microspheres were measured with two different system configurations and at different probe-sample distances. Experimental results showed very good agreement with theory after system-independent fluorescence intensity calibration.

© 2007 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

ToC Category:

Original Manuscript: January 17, 2007
Revised Manuscript: July 13, 2007
Manuscript Accepted: August 10, 2007
Published: October 4, 2007

Virtual Issues
Vol. 2, Iss. 11 Virtual Journal for Biomedical Optics

Jianhua Zhao, Harvey Lui, David I. McLean, and Haishan Zeng, "Toward instrument-independent quantitative measurement of fluorescence intensity in fiber-optic spectrometer systems," Appl. Opt. 46, 7132-7140 (2007)

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