Fluorescence lifetime imaging by using time-gated data acquisition
Applied Optics, Vol. 46, Issue 30, pp. 7384-7391 (2007)
http://dx.doi.org/10.1364/AO.46.007384
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Abstract
The use of the time gating technique for lifetime reconstruction in the Fourier domain is a novel technique. Time gating provides sufficient data points in the time domain for reliable application of the Fourier transform, which is essential for the time deconvolution of the system of the integral equations employed in the reconstruction. The Fourier domain telegraph equation is employed to model the light transport, which allows a sufficiently broad interval of frequencies to be covered. Reconstructed images contain enough information needed for recovering the lifetime distribution in a sample for any given frequency within the megahertz–gigahertz band. The use of this technique is essential for recovering time-dependent information in fluorescence imaging. This technique was applied in reconstruction of the lifetime distribution of four tubes filled with Rhodamine 6G embedded inside a highly scattering slab. Relatively accurate fluorescence lifetime reconstruction demonstrates the effectiveness and the potential of the proposed technique.
© 2007 Optical Society of America
OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(290.0290) Scattering : Scattering
(290.7050) Scattering : Turbid media
ToC Category:
Scattering
History
Original Manuscript: February 14, 2007
Revised Manuscript: July 4, 2007
Manuscript Accepted: July 23, 2007
Published: October 10, 2007
Virtual Issues
Vol. 2, Iss. 11 Virtual Journal for Biomedical Optics
Citation
Vadim Y. Soloviev, Khadija B. Tahir, James McGinty, Dan S. Elson, Mark A. A. Neil, Paul M. W. French, and Simon R. Arridge, "Fluorescence lifetime imaging by using time-gated data acquisition," Appl. Opt. 46, 7384-7391 (2007)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-46-30-7384
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