OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 46, Iss. 8 — Mar. 10, 2007
  • pp: 1343–1360

Comparison of spectral variation from spectroscopy to spectral imaging

Steven C. Gebhart, Shovan K. Majumder, and Anita Mahadevan-Jansen  »View Author Affiliations


Applied Optics, Vol. 46, Issue 8, pp. 1343-1360 (2007)
http://dx.doi.org/10.1364/AO.46.001343


View Full Text Article

Enhanced HTML    Acrobat PDF (2643 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Optical biopsy has been shown to discriminate between normal and diseased tissue with high sensitivity and specificity. Fiber-optic probe-based spectroscopy systems do not provide the necessary spatial information to guide therapy effectively, ultimately requiring a transition from probe-based spectroscopy to spectral imaging. The effect of such a transition on fluorescence and diffuse reflectance line shape is investigated. Inherent differences in spectral line shape between spectroscopy and imaging are characterized and many of these differences may be attributed to a shift in illumination–collection geometry between the two systems. Sensitivity of the line-shape disparity is characterized with respect to changes in sample absorption and scattering as well as to changes in various parameters of the fiber-optic probe design (e.g., fiber diameter, beam steering). Differences in spectral line shape are described in terms of the relative relationship between the light diffusion within the tissue and the distribution of source–detector separation distances for the probe-based and imaging illumination–collection geometries. Monte Carlo simulation is used to determine fiber configurations that minimize the line-shape disparity between the two systems. In conclusion, we predict that fiber-optic probe designs that mimic a spectral imaging geometry and spectral imaging systems designed to emulate a probe-based geometry will be difficult to implement, pointing toward a posteriori correction for illumination–collection geometry to reconcile imaging and probe-based spectral line shapes or independent evaluation of tissue discrimination accuracy for probe-based and spectral imaging systems.

© 2007 Optical Society of America

OCIS Codes
(110.7050) Imaging systems : Turbid media
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(260.2510) Physical optics : Fluorescence
(300.6170) Spectroscopy : Spectra

History
Original Manuscript: May 9, 2006
Revised Manuscript: September 13, 2006
Manuscript Accepted: November 2, 2006
Published: February 20, 2007

Virtual Issues
Vol. 2, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Steven C. Gebhart, Shovan K. Majumder, and Anita Mahadevan-Jansen, "Comparison of spectral variation from spectroscopy to spectral imaging," Appl. Opt. 46, 1343-1360 (2007)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-46-8-1343

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited