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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 47, Iss. 11 — Apr. 10, 2008
  • pp: 1922–1926

Detection of mechanical and disease stresses in citrus plants by fluorescence spectroscopy

J. Belasque, Jr., M. C. G. Gasparoto, and L. G. Marcassa  »View Author Affiliations

Applied Optics, Vol. 47, Issue 11, pp. 1922-1926 (2008)

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We have investigated the detection of mechanical and disease stresses in citrus plants (Citrus limonia [L.] Osbeck) using laser-induced fluorescence spectroscopy. Due to its economic importance we have chosen to investigate the citrus canker disease, which is caused by the Xanthomonas axonopodis pv. citri bacteria. Mechanical stress was also studied because it plays an important role in the plant’s infection by such bacteria. A laser-induced fluorescence spectroscopy system, composed of a spectrometer and a 532 nm 10 mW excitation laser was used to perform fluorescence spectroscopy. The ratio of two chlorophyll fluorescence bands allows us to detect and discriminate between mechanical and disease stresses. This ability to discriminate may have an important application in the field to detect citrus canker infected trees.

© 2008 Optical Society of America

OCIS Codes
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(300.2530) Spectroscopy : Fluorescence, laser-induced
(280.1415) Remote sensing and sensors : Biological sensing and sensors

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: September 27, 2007
Revised Manuscript: December 6, 2007
Manuscript Accepted: February 15, 2008
Published: April 7, 2008

Virtual Issues
Vol. 3, Iss. 5 Virtual Journal for Biomedical Optics

J. Belasque, Jr., M. C. G. Gasparoto, and L. G. Marcassa, "Detection of mechanical and disease stresses in citrus plants by fluorescence spectroscopy," Appl. Opt. 47, 1922-1926 (2008)

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