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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 47, Iss. 19 — Jul. 1, 2008
  • pp: D96–D109

Phase, amplitude, and polarization microscopy with a sampling field sensor

Remy Tumbar  »View Author Affiliations

Applied Optics, Vol. 47, Issue 19, pp. D96-D109 (2008)

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I describe an improved implementation of a previously reported interferometric device, the sampling field sensor (SFS) [ Appl. Opt. 47, B32–B43 (2008)]. It provides X, Y, and X Y shearing interferometric information simultaneously (space multiplexed) with amplitude and polarization information while using time-multiplexed phase shifting. Its simple common-path configuration makes it compact and vibration insensitive, as demonstrated by the λ / 125 phase estimation repeatability that was below the coherent noise floor (estimated at λ / 50 ). The SFS may be viewed as an efficient, robust and accurate full-field optical–digital interface, easy to integrate with traditional imaging systems. This is demonstrated by using the sensor as the focal plane array of a transmitted-light microscope in a straightforward setup using an illumination path polarization phase shifter. This work is focused on a qualitative demonstration and presents phase, amplitude, and polarization images of different types of human cheek cells and Caenorhabditiselegans larvae.

© 2008 Optical Society of America

OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(170.0180) Medical optics and biotechnology : Microscopy
(180.3170) Microscopy : Interference microscopy
(350.5030) Other areas of optics : Phase
(090.1995) Holography : Digital holography

Original Manuscript: October 1, 2007
Revised Manuscript: December 21, 2007
Manuscript Accepted: January 14, 2008
Published: March 19, 2008

Virtual Issues
Vol. 3, Iss. 8 Virtual Journal for Biomedical Optics

Remy Tumbar, "Phase, amplitude, and polarization microscopy with a sampling field sensor," Appl. Opt. 47, D96-D109 (2008)

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  1. A. V. Oppenheim and J. S. Lim, “The importance of phase in signals,” Proc. IEEE 69, 529-541 (1981). [CrossRef]
  2. J. Curlander and R. McDonough, Synthetic Aperture Radar: Systems and Signal Processing (Wiley, 1991).
  3. R. Tumbar, D. L. Marks, and D. J. Brady, “Robust, common-path, phase-shifting interferometer and optical profilometer,” Appl. Opt. 47, B32-B43 (2008). [CrossRef] [PubMed]
  4. D. Psaltis, “Coherent optical information systems,” Science 298, 1359-1363 (2002). [CrossRef] [PubMed]
  5. R. Tumbar and D. J. Brady, “Sampling field sensor with anisotropic fan-out,” Appl. Opt. 41, 6621-6636 (2002). [CrossRef] [PubMed]
  6. R. Tumbar and D. J. Brady, “Interferometric sensor and method to detect optical fields,” U.S. Patent 6,639,683 (2003).
  7. Handbook of Optics, M. Bass, ed. (McGraw-Hill, 1995), vol. 2.
  8. C. Rathjen, “Statistical properties of phase-shift algorithms,” J. Opt. Soc. Am. A 12, 1997-2008 (1995). [CrossRef]
  9. J. Schwider, R. Burow, K. E. Elssner, J. Grzanna, R. Spolaczyk, and K. Merkel, “Digital wave-front measuring interferometry: some systematic error sources,” Appl. Opt. 22, 3421-3432(1983). [CrossRef] [PubMed]
  10. P. Hariharan, B. F. Oreb, and T. Eiju, “Digital phase-shifting interferometry: a simple error-compensating phase calculation algorithm,” Appl. Opt. 26, 2504-2506 (1987). [CrossRef] [PubMed]
  11. S. S. Helen, M. P. Kothiyal, and R. S. Sirohi, “Achromatic phase shifting by a rotating polarizer,” Opt. Commun. 154, 249-254 (1998). [CrossRef]
  12. C. J. Cogswell, N. I. Smith, K. G. Larkin, and P. Hariharan, “Quantitative DIC microscopy using a geometric phase shifter,” Proc. SPIE 2984, 72-81 (1997). [CrossRef]
  13. K. Creath, “Phase-measurement interferometry techniques,” in Progress in Optics, E. Wolf, ed. (North-Holland, 1988), Vol. XXVI, p. 349. [CrossRef]
  14. J. W. Goodman, Introduction to Fourier Optics, 1st ed. (McGraw-Hill, 1968).
  15. D. C. Ghiglia and M. D. Pritt, Two-Dimensional Phase Unwrapping: Theory, Algorithms, and Software (Wiley, 1998).
  16. M. Laikin, Lens Design, 3rd ed. (Marcel Dekker, 2001).
  17. R. H. Dyck, “Multiple-frame CCD image sensor with overlying photosensitive layer,” U.S. Patent 5796433 (1998).
  18. T. Hori, “Method for making high-frame-rate CCD imaging devices from otherwise ordinary and inexpensive CCD devices,” U.S. Patent 6255134 (2001).
  19. R. Hard, R. Zeh, and R. D. Allen, “Phase-randomized laser illumination for microscopy,” J. Cell Sci. 23, 335-343 (1977). [PubMed]
  20. M. Shribak and R. Oldenbourg, “Techniques for fast and sensitive measurements of two-dimensional birefringence distributions,” Appl. Opt. 42, 3009-3017 (2003). [CrossRef] [PubMed]
  21. F. Zernike, “How I discovered phase contrast,” Science 121, 345-349 (1955). [CrossRef] [PubMed]
  22. G. Nomarski and A. R. Weill, “Application to metallography of interference methods with two waves in polarized light--application a la metallographie des methodes interferentielles a deux ondes polarisees,” Rev. Metall. 52, 121-134 (1955).
  23. G. Popescu, L. P. Deflores, J. C. Vaughan, K. Badizadegan, H. Iwai, R. R. Dasari, and M. S. Feld, “Fourier phase microscopy for investigation of biological structures and dynamics,” Opt. Lett. 29, 2503-2505 (2004). [CrossRef] [PubMed]
  24. D. Zicha, E. Genot, G. A. Dunn, and I. M. Kramer, “TGFbeta1 induces a cell-cycle-dependent increase in motility of epithelial cells,” J Cell Sci. 112, 447-454 (1999). [PubMed]
  25. H. Sun, M. A. Player, J. Watson, D. C. Hendry, R. G. Perkins, G. Gust, and D. M. Paterson, “The use of digital/electronic holography for biological applications,” J. Opt. A Pure Appl. Opt. 7, S399-S407 (2005). [CrossRef]
  26. W. J. Brown and M. G. Farquhar, “Accumulation of coated vesicles bearing mannose 6-phosphate receptors for lysosomal enzymes in the Golgi region of I-cell fibroblasts,” Proc. Natl. Acad. Sci. USA 81, 5135-5139 (1984). [CrossRef] [PubMed]
  27. F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J Biomed. Opt. 11, 054032 (2006). [CrossRef] [PubMed]
  28. P. Ferraro, D. Alferi, S. De Nicola, L. De Petrocellis, A. Finizio, and G. Pierattini, “Quantitative phase-contrast microscopy by a lateral shear approach to digital holographic image reconstruction,” Opt. Lett. 31, 1405-1407 (2006). [CrossRef] [PubMed]

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