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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 47, Iss. 24 — Aug. 20, 2008
  • pp: 4405–4412

Scanning flow cytometer modified to distinguish phytoplankton cells from their effective size, effective refractive index, depolarization, and fluorescence

Luca Fiorani, Valeri P. Maltsev, Vyacheslav M. Nekrasov, Antonio Palucci, Konstantin A. Semyanov, and Valeria Spizzichino  »View Author Affiliations


Applied Optics, Vol. 47, Issue 24, pp. 4405-4412 (2008)
http://dx.doi.org/10.1364/AO.47.004405


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Abstract

A laser flow cytometer based on scanning flow cytometry has been assembled. The unpolarized and linearly polarized light-scattering profiles, as well as the side emitted light in different spectral bands, were measured, allowing the simultaneous and real-time determination of the effective size and the effective refractive index of each spherelike particle. Additionally, each particle could be identified from depolarization and fluorescence measured simultaneously. The tests with aqueous samples of polystyrene spheres, fluorescent or nonfluorescent, and phytoplankton cells demonstrate that the system is able to retrieve size and refractive index with an accuracy of 1% and that the depolarization and fluorescence measurements allow the classification of particles otherwise indistinguishable.

© 2008 Optical Society of America

OCIS Codes
(120.5820) Instrumentation, measurement, and metrology : Scattering measurements
(170.1530) Medical optics and biotechnology : Cell analysis
(300.2530) Spectroscopy : Fluorescence, laser-induced

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: May 7, 2008
Manuscript Accepted: July 13, 2008
Published: August 19, 2008

Virtual Issues
Vol. 3, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Luca Fiorani, Valeri P. Maltsev, Vyacheslav M. Nekrasov, Antonio Palucci, Konstantin A. Semyanov, and Valeria Spizzichino, "Scanning flow cytometer modified to distinguish phytoplankton cells from their effective size, effective refractive index, depolarization, and fluorescence," Appl. Opt. 47, 4405-4412 (2008)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-47-24-4405

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