Abstract

We present studies of polarized absorption [linear dichroism (LD)] and fluorescence polarization of the styryl derivative (LDS 798) embedded in oriented poly(vinyl alcohol) (PVA) films. These films were oriented by progressive stretching up to eight folds. Both vertical and horizontal components of absorptions and fluorescence were measured and dichroic ratios were determined for different film stretching ratios. The dichroic ratio and fluorescence anisotropy values were analyzed as a function of PVA film stretching ratio by fitting according to the previously developed theory. For maximum stretching ratios, exceptionally high anisotropy ($\sim 0.8$) and polarization ($\sim 0.9$) values have been measured. The stretched films have high polarization values also for isotropic excitation in a wide spectral range ($500–700\mathrm{nm}$). Such films can be conveniently used as high polarization standards and we envision they will also have applications in near infrared (NIR) imaging microscopy, where they can be used for correcting an instrumental factor in polarization measurements.

© 2008 Optical Society of America

Polarized fluorescent nanospheres

Rafal Luchowski, Zygmunt Gryczynski, Zeno Földes-Papp, Aaron Chang, Julian Borejdo, Pabak Sarkar, and Ignacy Gryczynski
Opt. Express 18(5) 4289-4299 (2010)

Review of ultrafast fluorescence polarization spectroscopy [Invited]

Yang Pu, Wubao Wang, Richard B. Dorshow, Bidyut B. Das, and Robert R. Alfano
Appl. Opt. 52(5) 917-929 (2013)

Polarization distortion effects in polarimetric two-photon microscopy

Peter Schön, Fabiana Munhoz, Alicja Gasecka, Sophie Brustlein, and Sophie Brasselet
Opt. Express 16(25) 20891-20901 (2008)

Imaging of protein cluster sizes by means of confocal time-gated fluorescence anisotropy microscopy

Arjen N. Bader, Erik G. Hofman, Paul M. P. van Bergen en Henegouwen, and Hans C. Gerritsen
Opt. Express 15(11) 6934-6945 (2007)

Simultaneous widefield single molecule orientation and FRET microscopy in cells

S. E. D. Webb, D. J. Rolfe, S. R. Needham, S. K. Roberts, D. T. Clarke, C. I. McLachlan, M. P. Hobson, and M. L. Martin-Fernandez
Opt. Express 16(25) 20258-20265 (2008)