Digital holographic microscopy enables a quantitative phase contrast metrology that is suitable for the investigation of reflective surfaces as well as for the marker-free analysis of living cells. The digital holographic feature of (subsequent) numerical focus adjustment makes possible applications for multifocus imaging. An overview of digital holographic microscopy methods is described. Applications of digital holographic microscopy are demonstrated by results obtained from livings cells and engineered surfaces.
© 2008 Optical Society of America
Original Manuscript: May 8, 2007
Revised Manuscript: September 5, 2007
Manuscript Accepted: September 7, 2007
Published: October 26, 2007
Vol. 3, Iss. 3 Virtual Journal for Biomedical Optics
Björn Kemper and Gert von Bally, "Digital holographic microscopy for live cell applications and technical inspection," Appl. Opt. 47, A52-A61 (2008)