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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 48, Iss. 13 — May. 1, 2009
  • pp: 2595–2599

Confocal light scattering spectroscopic imaging system for in situ tissue characterization

Peter Huang, Martin Hunter, and Irene Georgakoudi  »View Author Affiliations

Applied Optics, Vol. 48, Issue 13, pp. 2595-2599 (2009)

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We report on the design and construction of a confocal light scattering spectroscopic imaging system aimed ultimately to conduct depth-resolved characterization of biological tissues. The confocal sectioning ability of the system is demonstrated using a two-layer sample consisting of a 200 μm thick cancer cell layer on top of a scattering layer doped with a green absorber. The measurement results demonstrate that distinct light scattering signals can be isolated from each layer with an axial and a lateral resolution of 30 and 27 μm , respectively. Such a system is expected to have significant applications in the areas of tissue engineering and disease diagnostics and monitoring.

© 2009 Optical Society of America

OCIS Codes
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(180.1790) Microscopy : Confocal microscopy
(290.1350) Scattering : Backscattering
(300.6550) Spectroscopy : Spectroscopy, visible

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: November 14, 2008
Revised Manuscript: March 28, 2009
Manuscript Accepted: April 3, 2009
Published: April 29, 2009

Virtual Issues
Vol. 4, Iss. 7 Virtual Journal for Biomedical Optics

Peter Huang, Martin Hunter, and Irene Georgakoudi, "Confocal light scattering spectroscopic imaging system for in situ tissue characterization," Appl. Opt. 48, 2595-2599 (2009)

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