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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 48, Iss. 20 — Jul. 10, 2009
  • pp: 3998–4002

Optimization of axial resolution in a confocal microscope with D-shaped apertures

Wei Gong, Ke Si, and Colin J. R. Sheppard  »View Author Affiliations


Applied Optics, Vol. 48, Issue 20, pp. 3998-4002 (2009)
http://dx.doi.org/10.1364/AO.48.003998


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Abstract

We show theoretically that the axial resolution is improved when two centrosymmetric D-shaped apertures are combined in a confocal microscope with a finite-sized pinhole. The optimum width of a divider that separates the D-shaped apertures to give the maximum axial resolution for a given pinhole size is investigated, and the magnitude of the signal level is explored.

© 2009 Optical Society of America

OCIS Codes
(050.1220) Diffraction and gratings : Apertures
(050.1970) Diffraction and gratings : Diffractive optics
(170.1530) Medical optics and biotechnology : Cell analysis
(290.0290) Scattering : Scattering

ToC Category:
Diffraction and Gratings

History
Original Manuscript: April 20, 2009
Revised Manuscript: June 19, 2009
Manuscript Accepted: June 23, 2009
Published: July 7, 2009

Virtual Issues
Vol. 4, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Wei Gong, Ke Si, and Colin J. R. Sheppard, "Optimization of axial resolution in a confocal microscope with D-shaped apertures," Appl. Opt. 48, 3998-4002 (2009)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-48-20-3998

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