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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 48, Iss. 23 — Aug. 10, 2009
  • pp: 4610–4615

Refractive index of dark-adapted bacteriorhodopsin and tris(hydroxymethyl)aminomethane buffer between 390 and 880 nm

Zsuzsanna Heiner and Károly Osvay  »View Author Affiliations


Applied Optics, Vol. 48, Issue 23, pp. 4610-4615 (2009)
http://dx.doi.org/10.1364/AO.48.004610


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Abstract

The refractivity of wild-type bacteriorhodopsin ( bR WT ) suspended in tris(hydroxymethyl)aminomethane (TRIS) buffer has been measured in the spectral range of 390 840 nm by the method of angle of minimal deviation with the use of a hollow glass prism. The refractive indices of pure bR WT as well as of TRIS buffer have been determined from the concentration dependent refraction values. Sellmeier-type dispersion equations have been fitted for both the TRIS buffer and pure bR WT .

© 2009 Optical Society of America

OCIS Codes
(160.4760) Materials : Optical properties
(160.1435) Materials : Biomaterials

ToC Category:
Materials

History
Original Manuscript: April 29, 2009
Revised Manuscript: July 10, 2009
Manuscript Accepted: July 25, 2009
Published: August 4, 2009

Citation
Zsuzsanna Heiner and Károly Osvay, "Refractive index of dark-adapted bacteriorhodopsin and tris(hydroxymethyl)aminomethane buffer between 390 and 880 nm," Appl. Opt. 48, 4610-4615 (2009)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-48-23-4610

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