OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Jospeh N. Mait
  • Vol. 48, Iss. 3 — Jan. 20, 2009
  • pp: 553–559

Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source

Pierre Blandin, Sandrine Lévêque-Fort, Sandrine Lécart, Jack C. Cossec, Marie-Claude Potier, Zsolt Lenkei, Frédéric Druon, and Patrick Georges  »View Author Affiliations


Applied Optics, Vol. 48, Issue 3, pp. 553-559 (2009)
http://dx.doi.org/10.1364/AO.48.000553


View Full Text Article

Enhanced HTML    Acrobat PDF (510 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We present the instrumental development of a versatile total internal reflection fluorescence lifetime imaging microscopy setup illuminated by a supercontinuum laser source. It enables performing wide-field fluorescence lifetime imaging with subwavelength axial resolution for a large range of fluorophores. The short overall acquisition time and the axial resolution are well suited for dynamic neurobiological applications.

© 2009 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(260.6970) Physical optics : Total internal reflection

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: June 20, 2008
Revised Manuscript: December 3, 2008
Manuscript Accepted: December 4, 2008
Published: January 14, 2009

Virtual Issues
Vol. 4, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Pierre Blandin, Sandrine Lévêque-Fort, Sandrine Lécart, Jack C. Cossec, Marie-Claude Potier, Zsolt Lenkei, Frédéric Druon, and Patrick Georges, "Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source," Appl. Opt. 48, 553-559 (2009)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-48-3-553

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited