In temporal phase-shifting-based digital holographic microscopy, high-resolution phase contrast imaging requires optimized conditions for hologram recording and phase retrieval. To optimize the phase resolution, for the example of a variable three-step algorithm, a theoretical analysis on statistical errors, digitalization errors, uncorrelated errors, and errors due to a misaligned temporal phase shift is carried out. In a second step the theoretically predicted results are compared to the measured phase noise obtained from comparative experimental investigations with several coherent and partially coherent light sources. Finally, the applicability for noise reduction is demonstrated by quantitative phase contrast imaging of pancreas tumor cells.
© 2009 Optical Society of America
Original Manuscript: September 22, 2008
Revised Manuscript: January 16, 2009
Manuscript Accepted: February 7, 2009
Published: March 3, 2009
Vol. 4, Iss. 5 Virtual Journal for Biomedical Optics
Christian Remmersmann, Stephan Stürwald, Björn Kemper, Patrik Langehanenberg, and Gert von Bally, "Phase noise optimization in temporal phase-shifting digital holography with partial coherence light sources and its application in quantitative cell imaging," Appl. Opt. 48, 1463-1472 (2009)