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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 49, Iss. 16 — Jun. 1, 2010
  • pp: D145–D156

Assessment of a liquid lens enabled in vivo optical coherence microscope

Supraja Murali, Panomsak Meemon, Kye-Sung Lee, William P. Kuhn, Kevin P. Thompson, and Jannick P. Rolland  »View Author Affiliations


Applied Optics, Vol. 49, Issue 16, pp. D145-D156 (2010)
http://dx.doi.org/10.1364/AO.49.00D145


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Abstract

The optical aberrations induced by imaging through skin can be predicted using formulas for Seidel aberrations of a plane-parallel plate. Knowledge of these aberrations helps to guide the choice of numerical aperture (NA) of the optics we can use in an implementation of Gabor domain optical coherence microscopy (GD-OCM), where the focus is the only aberration adjustment made through depth. On this basis, a custom-designed, liquid-lens enabled dynamic focusing optical coherence microscope operating at 0.2 NA is analyzed and validated experimentally. As part of the analysis, we show that the full width at half-maximum metric, as a characteristic descriptor for the point spread function, while commonly used, is not a useful metric for quantifying resolution in non-diffraction-limited systems. Modulation transfer function (MTF) measurements quantify that the liquid lens performance is as predicted by design, even when accounting for the effect of gravity. MTF measurements in a skinlike scattering medium also quantify the performance of the microscope in its potential applications. To guide the fusion of images across the various focus positions of the microscope, as required in GD-OCM, we present depth of focus measurements that can be used to determine the effective number of focusing zones required for a given goal resolution. Subcellular resolution in an onion sample, and high-definition in vivo imaging in human skin are demonstrated with the custom-designed and built microscope.

© 2010 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(180.1655) Microscopy : Coherence tomography
(220.1080) Optical design and fabrication : Active or adaptive optics

ToC Category:
Microscopy

History
Original Manuscript: November 3, 2009
Revised Manuscript: March 5, 2010
Manuscript Accepted: March 18, 2010
Published: May 28, 2010

Virtual Issues
Vol. 5, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Supraja Murali, Panomsak Meemon, Kye-Sung Lee, William P. Kuhn, Kevin P. Thompson, and Jannick P. Rolland, "Assessment of a liquid lens enabled in vivooptical coherence microscope," Appl. Opt. 49, D145-D156 (2010)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-49-16-D145

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