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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 49, Iss. 26 — Sep. 10, 2010
  • pp: 4890–4897

Ultraviolet analysis of donated corneas: a portable prototype

Victor A. C. Lincoln, Liliane Ventura, and Sidney J. Faria e Sousa  »View Author Affiliations

Applied Optics, Vol. 49, Issue 26, pp. 4890-4897 (2010)

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As technology improves human vision, some procedures currently performed may be causing a decrease of the natural UV protection of the cornea. A portable dual beam system prototype was assembled for physicians for clinical studies of these effects on the corneas endowing two types of 300–400 nm evaluations: 1, regularly donated corneas and 2, simulating refractive keratectomy by corneal lamellae removal. The system performs 500 measurements/s, providing ± 0.25 % precision for the transmittance. The measurements performed on the prototype are 95% in agreement with Cary 17 and HR4000CG-UV-NIR Ocean Optics spectrophotometers. Preliminary studies on cadaveric corneas demonstrate that, as the stromal layer is reduced ( 150 μm depth), there is significant loss—an average of 7.1%.—of the cornea’s natural UV protection. The prototype is being tested in an eye bank for routine evaluation of donor corneas.

© 2010 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(300.6540) Spectroscopy : Spectroscopy, ultraviolet
(330.5380) Vision, color, and visual optics : Physiology

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: February 4, 2010
Revised Manuscript: July 14, 2010
Manuscript Accepted: August 4, 2010
Published: September 3, 2010

Virtual Issues
Vol. 5, Iss. 13 Virtual Journal for Biomedical Optics

Victor A. C. Lincoln, Liliane Ventura, and Sidney J. Faria e Sousa, "Ultraviolet analysis of donated corneas: a portable prototype," Appl. Opt. 49, 4890-4897 (2010)

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