Changes in optical properties of rat cerebral cortical slices during oxygen glucose deprivation

Izumi Nishidate; Keiichiro Yoshida; Manabu Sato

doi:10.1364/AO.49.006617

Applied Optics
Vol. 49,
Issue 34,
pp. 6617-6623
(2010)
•https://doi.org/10.1364/AO.49.006617

Changes in optical properties of rat cerebral cortical slices during oxygen glucose deprivation

Izumi Nishidate, Keiichiro Yoshida, and Manabu Sato

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Izumi Nishidate, Keiichiro Yoshida, and Manabu Sato, "Changes in optical properties of rat cerebral cortical slices during oxygen glucose deprivation," Appl. Opt. 49, 6617-6623 (2010)

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- Medical Optics and Biotechnology
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About this Article
History
- Original Manuscript: May 18, 2010
- Revised Manuscript: October 13, 2010
- Manuscript Accepted: October 20, 2010
- Published: November 24, 2010

Abstract

We simultaneously measured the diffuse reflectance spectra and transmittance spectra of in vitro rat cerebral cortical tissue slices perfused with artificial cerebrospinal fluid (aCSF) in the wavelength range from 500 to $900 nm$ . An ischemia-like condition in the cortical tissue was induced by oxygen/glucose deprivation (OGD) of the aCSF. Diffuse reflectance and transmittance of the cortical slices were decreased and increased, respectively, during OGD. Spectral data of reduced scattering coefficients and absorption coefficients were estimated by the inverse Monte Carlo simulation for light transport in tissue. As with OGD, significant decrease of the reduced scattering coefficients and alteration of the absorption coefficient spectrum were observed over the measured wavelength range. The mean maximum amplitudes of change in the absorption coefficient at 520, 550, 605, and $830 nm$ were $0.33 \pm 0.14$ , $0.30 \pm 0.12$ , $0.30 \pm 0.14$ , and $- 0.04 \pm 0.16$ , respectively, whereas those in the reduced scattering coefficient at 520, 550, 605, and $830 nm$ were $- 0.37 \pm 0.08$ , $- 0.38 \pm 0.08$ , $- 0.38 \pm 0.08$ , and $- 0.39 \pm 0.08$ . Variations in the reduced scattering coefficients implied cell deformation mainly due to cell swelling, whereas those in the absorption spectra indicated reductions in heme $a a_{3}$ and CuA in cytochrome c oxidase and cytochrome c.

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λ (nm)	${μ_{s}}^{'}$ ( ${cm}^{- 1}$ )		$μ_{a}$ ( ${cm}^{- 1}$ )
	Normal	OGD	Normal	OGD
500	$43.7 \pm 9.2$	$29.4 \pm 7.6$	$4.8 \pm 1.2$	$6.1 \pm 1.5$
510	$42.6 \pm 8.7$	$28.3 \pm 7.4$	$5.0 \pm 1.2$	$6.3 \pm 1.5$
520	$41.2 \pm 8.4$	$27.5 \pm 6.9$	$5.3 \pm 1.2$	$6.6 \pm 1.5$
530	$39.6 \pm 8.0$	$26.5 \pm 6.6$	$5.3 \pm 1.2$	$6.6 \pm 1.5$
540	$38.6 \pm 7.4$	$25.6 \pm 6.4$	$5.5 \pm 1.2$	$6.7 \pm 1.4$
550	$38.0 \pm 7.1$	$25.2 \pm 6.0$	$5.8 \pm 1.2$	$7.2 \pm 1.4$
560	$36.7 \pm 7.1$	$24.4 \pm 5.9$	$5.7 \pm 1.2$	$6.9 \pm 1.4$
570	$35.2 \pm 6.6$	$23.4 \pm 5.6$	$5.4 \pm 1.1$	$6.5 \pm 1.3$
580	$34.2 \pm 6.2$	$22.6 \pm 5.3$	$5.2 \pm 1.1$	$6.3 \pm 1.3$
590	$33.2 \pm 6.3$	$21.9 \pm 5.1$	$5.0 \pm 1.1$	$6.1 \pm 1.2$
600	$32.0 \pm 5.9$	$21.1 \pm 4.9$	$4.9 \pm 1.0$	$5.9 \pm 1.2$
605	$31.5 \pm 5.8$	$20.8 \pm 4.8$	$4.9 \pm 1.0$	$5.9 \pm 1.2$
610	$31.1 \pm 5.5$	$20.4 \pm 4.7$	$4.8 \pm 1.0$	$5.7 \pm 1.2$
620	$29.8 \pm 5.3$	$19.6 \pm 4.4$	$4.5 \pm 1.0$	$5.4 \pm 1.1$
630	$28.8 \pm 5.0$	$18.9 \pm 4.2$	$4.4 \pm 1.0$	$5.2 \pm 1.1$
640	$28.0 \pm 5.0$	$18.2 \pm 4.0$	$4.4 \pm 0.9$	$5.1 \pm 1.0$
650	$27.2 \pm 4.7$	$17.8 \pm 3.8$	$4.4 \pm 0.9$	$5.0 \pm 1.0$
660	$26.4 \pm 4.4$	$17.1 \pm 3.6$	$4.3 \pm 0.9$	$4.9 \pm 1.0$
670	$25.6 \pm 4.2$	$16.7 \pm 3.5$	$4.3 \pm 0.9$	$4.8 \pm 1.0$
680	$24.8 \pm 4.1$	$16.3 \pm 3.3$	$4.3 \pm 0.9$	$4.8 \pm 1.0$
690	$24.1 \pm 3.9$	$15.8 \pm 3.2$	$4.2 \pm 0.9$	$4.7 \pm 0.9$
700	$23.5 \pm 3.8$	$15.3 \pm 3.0$	$4.2 \pm 0.9$	$4.6 \pm 1.0$
710	$23.0 \pm 3.5$	$14.9 \pm 2.8$	$4.2 \pm 0.9$	$4.6 \pm 1.0$
720	$22.3 \pm 3.4$	$14.6 \pm 2.7$	$4.1 \pm 0.9$	$4.5 \pm 1.0$
730	$21.7 \pm 3.3$	$14.2 \pm 2.6$	$4.1 \pm 0.9$	$4.4 \pm 0.9$
740	$21.3 \pm 3.1$	$13.9 \pm 2.4$	$4.1 \pm 0.9$	$4.4 \pm 1.0$
750	$20.8 \pm 3.1$	$13.5 \pm 2.4$	$4.1 \pm 0.9$	$4.3 \pm 0.9$
760	$20.3 \pm 3.0$	$13.2 \pm 2.2$	$4.0 \pm 1.0$	$4.2 \pm 0.9$
770	$19.9 \pm 2.8$	$12.9 \pm 2.2$	$4.0 \pm 0.9$	$4.2 \pm 0.9$
780	$19.3 \pm 2.8$	$12.6 \pm 2.1$	$3.9 \pm 1.0$	$4.0 \pm 1.0$
790	$19.0 \pm 2.6$	$12.4 \pm 2.0$	$3.9 \pm 0.9$	$4.0 \pm 0.9$
800	$18.5 \pm 2.5$	$12.1 \pm 1.8$	$3.9 \pm 1.0$	$3.8 \pm 0.9$
810	$18.2 \pm 2.5$	$11.8 \pm 1.7$	$3.8 \pm 1.0$	$3.8 \pm 0.9$
820	$17.9 \pm 2.4$	$11.7 \pm 1.7$	$3.7 \pm 1.0$	$3.7 \pm 0.9$
830	$17.6 \pm 2.4$	$11.4 \pm 1.7$	$3.7 \pm 0.9$	$3.6 \pm 0.9$
840	$17.2 \pm 2.2$	$11.3 \pm 1.7$	$3.7 \pm 0.9$	$3.6 \pm 1.0$
850	$16.9 \pm 2.2$	$11.1 \pm 1.4$	$3.6 \pm 1.0$	$3.4 \pm 0.9$
860	$16.7 \pm 2.2$	$11.1 \pm 1.5$	$3.5 \pm 1.0$	$3.4 \pm 1.0$
870	$16.6 \pm 2.1$	$10.9 \pm 1.5$	$3.5 \pm 0.9$	$3.3 \pm 0.9$
880	$16.4 \pm 2.1$	$10.8 \pm 1.4$	$3.4 \pm 0.9$	$3.2 \pm 0.9$
890	$16.4 \pm 2.1$	$10.7 \pm 1.3$	$3.3 \pm 1.0$	$3.1 \pm 0.9$
900	$16.0 \pm 2.0$	$10.6 \pm 1.3$	$3.2 \pm 1.0$	$2.9 \pm 0.9$

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Applied Optics