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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 49, Iss. 5 — Feb. 10, 2010
  • pp: 887–891

High resolution imaging of patterned model biological membranes by localized surface plasmon microscopy

Koyo Watanabe, Miyazaki Ryosuke, Goro Terakado, Takashi Okazaki, Kenichi Morigaki, and Hiroshi Kano  »View Author Affiliations


Applied Optics, Vol. 49, Issue 5, pp. 887-891 (2010)
http://dx.doi.org/10.1364/AO.49.000887


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Abstract

We report on microscopic imaging of phospholipid membranes. To achieve nonlabel, noncontact, and high spatial resolution imaging of the membranes, we use optically excited localized surface plasmons as a virtual measurement probe to obtain the local refractive index. This enables significantly higher lateral resolution of 170 nm . We reveal that the developed microscope has the capability of observing lipid bilayers with thickness of 3.0 nm deposited into the gaps in a patterned lipid bilayer with thickness of 4.6 nm . We find that the thickness resolution against the deposited lipid bilayer is 0.33 nm .

© 2010 Optical Society of America

OCIS Codes
(120.6650) Instrumentation, measurement, and metrology : Surface measurements, figure
(180.0180) Microscopy : Microscopy
(240.6680) Optics at surfaces : Surface plasmons

ToC Category:
Microscopy

History
Original Manuscript: August 4, 2009
Revised Manuscript: January 14, 2010
Manuscript Accepted: January 14, 2010
Published: February 4, 2010

Virtual Issues
Vol. 5, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Koyo Watanabe, Miyazaki Ryosuke, Goro Terakado, Takashi Okazaki, Kenichi Morigaki, and Hiroshi Kano, "High resolution imaging of patterned model biological membranes by localized surface plasmon microscopy," Appl. Opt. 49, 887-891 (2010)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-49-5-887


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References

  1. E. Kretschmann and H. Raether, “Radiative decay of nonradiative surface plasmons excited by light,” Z. Naturforsch. A 23, 2135-2136 (1968).
  2. B. Rothenhäusler, C. Duschl, and W. Knoll, “Plasmon surface polariton fields for the characterization of thin films,” Thin Solid Films 159, 323-330 (1988). [CrossRef]
  3. W. Hickel and W. Knoll, “Surface plasmon microscopy of lipid layers,” Thin Solid Films 187, 349-356 (1990). [CrossRef]
  4. E. Sackmann, “Supported membranes: scientific and practical applications,” Science 271, 43-48 (1996). [CrossRef] [PubMed]
  5. J. T. Groves and S. G. Boxer, “Micropattern formation in supported lipid membranes,” Acc. Chem. Res. 35, 149-157 (2002). [CrossRef] [PubMed]
  6. K. Tawa and K. Morigaki, “Substrate-supported phospholipid membranes studied by surface plasmon resonance and surface plasmon fluorescence spectroscopy,” Biophys. J. 89, 2750-2758 (2005). [CrossRef] [PubMed]
  7. C. A. Keller and B. Kasemo, “Surface specific kinetics of lipid vesicle adsorption measured with a quartz crystal microbalance,” Biophys. J. 75, 1397-1402 (1998). [CrossRef] [PubMed]
  8. A. T. A. Jenkins, T. Neumann, and A. Offenhäusser, “Surface plasmon microscopy measurements of lipid vesicle adsorption on a micropatterned self-assembled monolayer,” Langmuir 17, 265-267 (2001). [CrossRef]
  9. Z. Z. Wang, T. Wilkop, and Q. Cheng, “Characterization of micropatterned lipid membranes on a gold surface by surface plasmon resonance imaging and electrochemical signaling of a pore-forming protein,” Langmuir 21, 10292-10296 (2005). [CrossRef] [PubMed]
  10. C. E. H. Berger, R. P. H. Kooyman, and J. Greve, “Resolution in surface plasmon microscopy,” Rev. Sci. Instrum. 65, 2829-2836 (1994). [CrossRef]
  11. H. Raether, Surface Plasmons on Smooth and Rough Surfaces and on Gratings (Springer, 1988).
  12. H. Kano, S. Mizuguchi, and S. Kawata, “Excitation of surface plasmon polaritons by a focused laser beam,” J. Opt. Soc. Am. B 15, 1381-1386 (1998). [CrossRef]
  13. Q. Zhan, “Evanescent Bessel beam generation via surface plasmon resonance excitation by a radially polarized beam,” Opt. Lett. 31, 1726-1728 (2006). [CrossRef] [PubMed]
  14. H. Kano and W. Knoll, “A scanning microscope employing localized surface-plasmon-polaritons as a sensing probe,” Opt. Commun. 182, 11-15 (2000). [CrossRef]
  15. K. Watanabe, N. Horiguchi, and H. Kano, “Optimized measurement probe of the localized surface plasmon microscope by using radially polarized illumination,” Appl. Opt. 46, 4985-4990 (2007). [CrossRef] [PubMed]
  16. K. Morigaki, T. Baumgart, A. Offenhäusser, and W. Knoll, “Patterning solid-supported lipid bilayer membranes by lithographic polymerization of a diacetylene lipid,” Angew. Chem. Int. Ed. Engl. 40, 172-174 (2001). [CrossRef] [PubMed]
  17. H. Kano and W. Knoll, “Locally excited surface-plasmon-polaritons for thickness measurement of LBK films,” Opt. Commun. 153, 235-239 (1998). [CrossRef]
  18. G. Miyaji, K. Ohbayashi, K. Sueda, K. Tsubakimoto, and N. Miyanaga, “Generation of vector beams with axially symmetric polarization,” Rev. Laser Eng. 32, 259-264 (2004). [CrossRef]
  19. G. Terakado, K. Watanabe, and H. Kano, “Scanning confocal total internal reflection fluorescence microscopy by using radial polarization in the illumination system,” Appl. Opt. 48, 1114-1118 (2009). [CrossRef]
  20. K. Morigaki, H. Schönherr, and T. Okazaki, “Polymerization of diacetylen phospholipid bilayers on solid substrate: influence of the film deposition temperature,” Langmuir 23, 12254-12260 (2007). [CrossRef] [PubMed]
  21. D. Marsh, CRC Handbook of Lipid Bilayers (CRC Press, 1990).

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