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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 49, Iss. 5 — Feb. 10, 2010
  • pp: 887–891

High resolution imaging of patterned model biological membranes by localized surface plasmon microscopy

Koyo Watanabe, Miyazaki Ryosuke, Goro Terakado, Takashi Okazaki, Kenichi Morigaki, and Hiroshi Kano  »View Author Affiliations

Applied Optics, Vol. 49, Issue 5, pp. 887-891 (2010)

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We report on microscopic imaging of phospholipid membranes. To achieve nonlabel, noncontact, and high spatial resolution imaging of the membranes, we use optically excited localized surface plasmons as a virtual measurement probe to obtain the local refractive index. This enables significantly higher lateral resolution of 170 nm . We reveal that the developed microscope has the capability of observing lipid bilayers with thickness of 3.0 nm deposited into the gaps in a patterned lipid bilayer with thickness of 4.6 nm . We find that the thickness resolution against the deposited lipid bilayer is 0.33 nm .

© 2010 Optical Society of America

OCIS Codes
(120.6650) Instrumentation, measurement, and metrology : Surface measurements, figure
(180.0180) Microscopy : Microscopy
(240.6680) Optics at surfaces : Surface plasmons

ToC Category:

Original Manuscript: August 4, 2009
Revised Manuscript: January 14, 2010
Manuscript Accepted: January 14, 2010
Published: February 4, 2010

Virtual Issues
Vol. 5, Iss. 5 Virtual Journal for Biomedical Optics

Koyo Watanabe, Miyazaki Ryosuke, Goro Terakado, Takashi Okazaki, Kenichi Morigaki, and Hiroshi Kano, "High resolution imaging of patterned model biological membranes by localized surface plasmon microscopy," Appl. Opt. 49, 887-891 (2010)

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