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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 50, Iss. 1 — Jan. 1, 2011
  • pp: 95–102

Errors in confocal fluorescence ratiometric imaging microscopy due to chromatic aberration

Yuxiang Lin and Arthur F. Gmitro  »View Author Affiliations


Applied Optics, Vol. 50, Issue 1, pp. 95-102 (2011)
http://dx.doi.org/10.1364/AO.50.000095


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Abstract

Confocal fluorescence ratiometric imaging is an optical technique used to measure a variety of important biological parameters. A small amount of chromatic aberration in the microscope system can introduce a variation in the signal ratio dependent on the fluorophore concentration gradient along the optical axis and lead to bias in the measurement. We present a theoretical model of this effect. Experimental results and simulations clearly demonstrate that this error can be significant and should not be ignored.

© 2011 Optical Society of America

OCIS Codes
(080.1010) Geometric optics : Aberrations (global)
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy

ToC Category:
Microscopy

History
Original Manuscript: July 12, 2010
Revised Manuscript: November 5, 2010
Manuscript Accepted: November 5, 2010
Published: December 27, 2010

Virtual Issues
Vol. 6, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Yuxiang Lin and Arthur F. Gmitro, "Errors in confocal fluorescence ratiometric imaging microscopy due to chromatic aberration," Appl. Opt. 50, 95-102 (2011)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-50-1-95

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