OSA's Digital Library

Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 50, Iss. 10 — Apr. 1, 2011
  • pp: 1389–1395

Early detection of liver cancer based on bioluminescence tomography

Xibo Ma, Jie Tian, Chenghu Qin, Xin Yang, Bo Zhang, Zhenwen Xue, Xing Zhang, Dong Han, Di Dong, and Xueyan Liu  »View Author Affiliations

Applied Optics, Vol. 50, Issue 10, pp. 1389-1395 (2011)

View Full Text Article

Enhanced HTML    Acrobat PDF (570 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



As a new modality of molecular imaging, bioluminescence imaging has been widely used in tumor detection and drug evaluation. However, BLI cannot present the depth of information for internal diseases such as a liver tumor in situ or a lung tumor in situ. In this paper, we describe a bioluminescence tomography (BLT) method based on the bioluminescent intensity attenuation calibration and applied it to the early detection of liver cancer in situ. In comparison with BLT without calibration, this method could improve the reconstruction accuracy by more than 10%. In comparison with micro-computed tomography and other traditional imaging modalities, this method can detect a liver tumor at a very early stage and provide reliable location information.

© 2011 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1420) Medical optics and biotechnology : Biology
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6960) Medical optics and biotechnology : Tomography

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: October 14, 2010
Revised Manuscript: January 28, 2011
Manuscript Accepted: January 28, 2011
Published: March 28, 2011

Virtual Issues
Vol. 6, Iss. 5 Virtual Journal for Biomedical Optics

Xibo Ma, Jie Tian, Chenghu Qin, Xin Yang, Bo Zhang, Zhenwen Xue, Xing Zhang, Dong Han, Di Dong, and Xueyan Liu, "Early detection of liver cancer based on bioluminescence tomography," Appl. Opt. 50, 1389-1395 (2011)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. J. Willmann, N. Bruggen, L. Dinkelborg, and S. Gambhir, “Molecular imaging in drug development,” Nature 7, 591–606(2008). [CrossRef]
  2. R. Weissleder and V. Ntziachristos, “Shedding light onto live molecular targets,” Nat. Med. 9, 123–128 (2003). [CrossRef] [PubMed]
  3. V. Ntziachristos, J. Ripoll, L. H. V. Wang, and R. Weissleder, “Looking and listening to light: the evolution of whole-body photonic imaging,” Nat. Biotechnol. 23, 313–320 (2005). [CrossRef] [PubMed]
  4. R. Paulmurugan and S. Gambhir, “Monitoring protein-protein interactions using split synthetic renilla luciferase protein-fragment-assisted complementation,” Anal. Chem. 75, 1584–1589 (2003). [CrossRef] [PubMed]
  5. K. Luker, M. Smith, G. Luker, S. Gammon, H. Piwnica-Worms, and D. Piwnica-Worms, “Kinetics of regulated protein-protein interactions revealed with firefly luciferase complementation imaging in cells and living animals,” Proc. Natl. Acad. Sci. USA 101, 12288–12293 (2004). [CrossRef] [PubMed]
  6. R. Paulmurugan and S. Gambhir, “Novel fusion protein approach for efficient high-throughput screening of small molecule-mediating protein-protein interactions in cells and living animals,” Cancer Res. 65, 7413–7420 (2005). [CrossRef] [PubMed]
  7. I. Remy and S. Michnick, “A highly sensitive protein-protein interaction assay based on Gaussia luciferase,” Nat. Methods 3, 977–979 (2006). [CrossRef] [PubMed]
  8. M. Funovics, R. Weissleder, and C. Tung, “Protease sensors for bioimaging,” Anal. Bioanal. Chem. 377, 956–963 (2003). [CrossRef] [PubMed]
  9. J. Grimm, D. Kirsch, and D. Windsor, “Use of gene expression profiling to direct in vivo molecular imaging of lung cancer,” Proc. Natl. Acad. Sci. USA 102, 14404–14409 (2005). [CrossRef] [PubMed]
  10. T. Jiang, E. Olson, Q. Nguyen, M. Roy, P. Jennings, and R. Tsien, “Tumor imaging by means of proteolytic activation of cell-penetrating peptides,” Proc. Natl. Acad. Sci. USA 101, 17867–17872 (2004). [CrossRef] [PubMed]
  11. G. Luker, C. Pica, J. Song, K. Luker, and D. Piwnica-Worms, “Imaging 26S proteasome activity and inhibition in living mice,” Nat. Med. 9, 969–973 (2003). [CrossRef] [PubMed]
  12. G. Zhang, M. Safran, and W. Wei, “Bioluminescent imaging of Cdk2 inhibition in vivo,” Nat. Med. 10, 643–648 (2004). [CrossRef] [PubMed]
  13. M. Safran, W. Kim, and F. O’Connell, “Mouse model for noninvasive imaging of HIF prolyl hydroxylase activity: assessment of an oral agent that stimulates erythropoietin production,” Proc. Natl. Acad. Sci. USA 103, 105–110 (2006). [CrossRef]
  14. X. Ma, Z. Liu, J. Tian, and F. Wang, “Dual-modality monitoring of tumor response to cyclophosphamide therapy in mice with bioluminescence imaging and small-animal positron emission tomography,” Mol. Imaging (to be published). [PubMed]
  15. Y. Lv, J. Tian, W. Cong, G. Wang, J. Luo, W. Yang, and H. Li, “A multilevel adaptive finite element algorithm for bioluminescence tomography,” Opt. Express 14, 8211–8223 (2006). [CrossRef] [PubMed]
  16. G. Wang, H. Shen, and W. Cong, “Temperature-modulated bioluminescence tomography,” Opt. Express 14, 7852–7871(2006). [CrossRef] [PubMed]
  17. W. Cong and K. Durairaj, “A Born-type approximation method for bioluminescence tomography,” Med. Phys. 33, 679–686(2006). [CrossRef] [PubMed]
  18. B. Zhang, X. Yang, C. Qin, D. Liu, S. Zhu, J. Feng, and J. Tian, “A trust region method in adaptive finite element framework for bioluminescence tomography,” Opt. Express 18, 6477–6491(2010). [CrossRef] [PubMed]
  19. J. Feng, K. Jia, C. Qin, G. Yan, S. Zhu, X. Zhang, J. Liu, and J. Tian, “Three-dimensional bioluminescence tomography based on Bayesian approach,” Opt. Express 17, 16834–16848(2009). [CrossRef] [PubMed]
  20. K. Liu, J. Tian, X. Yang, Y. Lu, C. Qin, S. Zhu, and X. Zhang, “A fast bioluminescent source localization method based on generalized graph cuts with mouse model validations,” Opt. Express 18, 3732–3745 (2010). [CrossRef] [PubMed]
  21. G. Wang, W. Cong, K. Durairaj, and M. Henry, “In vivo mouse studies with bioluminescence tomography,” Opt. Express 14, 7801–7809 (2006). [CrossRef] [PubMed]
  22. X. Qu, X. Li, X. Ma, R. Han, Z. Hu, X. Chen, D. Sun, R. Zhang, D. Chen, D. Chen, X. Chen, J. Liang, F. Cao, and J. Tian, “In vivo quantitative bioluminescence tomography using heterogenous and homogeneous mouse models,” Opt. Express 18, 13102–13113 (2010). [CrossRef] [PubMed]
  23. J. Zhao, L. Dong, and B. Lu, “Down-regulation of osteopontin suppresses growth and metastasis of hepatocellular carcinoma via induction of apoptosis,” Gastroenterology 135, 956–968 (2008). [CrossRef] [PubMed]
  24. J. Zhao, B. Lu, and H. Xu, “Thirty-kilodalton Tat-interacting protein suppresses tumor metastasis by inhibition of osteopontin transcription in human hepatocellular carcinoma,” Hepatology 48, 265–275 (2008). [CrossRef] [PubMed]
  25. J. Tian, J. Xue, and Y. Dai, “A novel software platform for medical image processing and analyzing,” IEEE Trans. Inf. Technol. Biomed. 12, 800–812 (2008). [CrossRef] [PubMed]
  26. G. Yan, J. Tian, S. Zhu, Y. Dai, and C. Qin, “Fast cone beam CT image reconstruction using GPU hardware,” J. X-Ray Sci. Technol. 16, 225–234 (2008), http://www.3dmed.net/paper/YanGR_XRay_Fast%20cone-beam%20CT%20image%20reconstruction%20using%20GPU%20hardware.pdf.
  27. G. Alexandrakis, F. Rannou, and A. Chatziioannou, “Tomographic bioluminescence imaging by use of a combined optical-PET (OPET) system: a computer simulation feasibility study,” Phys. Med. Biol. 50, 4225–4242 (2005). [CrossRef] [PubMed]
  28. M. Schweiger, S. R. Arridge, M. Hiraoka, and D. T. Delpy, “The finite element method for the propagation of light in scattering media: boundary and source conditions,” Med. Phys. 22, 1779–1792 (1995). [CrossRef] [PubMed]
  29. W. Cong, G. Wang, D. Kumar, Y. Liu, M. Jiang, L. V. Wang, E. A. Hoffman, G. McLennan, P. B. McCray, J. Zabner, and A. Cong, “Practical reconstruction method for bioluminescence tomography,” Opt. Express 13, 6756–6771(2005). [CrossRef] [PubMed]
  30. S. S. Rao, The Finite Element Method in Engineering(Butterworth-Heinemann, 1999).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited