Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Simple way of pinpointing the three-dimensional position of biomarkers in fluorescence microscopy using a through-focus exposure method

Not Accessible

Your library or personal account may give you access

Abstract

The author proposes a method to identify the three-dimensional positions of fluorescent biomarkers by recording just two images. In the proposed method, the x and y positions of all fluorescent markers are recorded in the first exposure, and the z positions are obtained from a blurred image in the second exposure. The author has verified this method using a specimen with 1μm deep grooves and applied it to measuring chromatic aberration and the separation between two biological probes in fluorescence in situ hybridization cells. The method offers the advantage of greatly reduced data storage requirements.

© 2011 Optical Society of America

Full Article  |  PDF Article
More Like This
Fluorescence microscopy three-dimensional depth variant point spread function interpolation using Zernike moments

Elie Maalouf, Bruno Colicchio, and Alain Dieterlen
J. Opt. Soc. Am. A 28(9) 1864-1870 (2011)

Three-dimensional holographic fluorescence microscopy

Bradley W. Schilling, Ting-Chung Poon, Guy Indebetouw, Brian Storrie, K. Shinoda, Y. Suzuki, and Ming Hsien Wu
Opt. Lett. 22(19) 1506-1508 (1997)

Three-dimensional positioning of optically trapped nanoparticles

Takayuki Higuchi, Quang Duc Pham, Satoshi Hasegawa, and Yoshio Hayasaki
Appl. Opt. 50(34) H183-H188 (2011)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (15)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (6)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved