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Simple way of pinpointing the three-dimensional position of biomarkers in fluorescence microscopy using a through-focus exposure method |
Applied Optics, Vol. 50, Issue 25, pp. 4989-4997 (2011)
http://dx.doi.org/10.1364/AO.50.004989
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Abstract
The author proposes a method to identify the three-dimensional positions of fluorescent biomarkers by recording just two images. In the proposed method, the x and y positions of all fluorescent markers are recorded in the first exposure, and the z positions are obtained from a blurred image in the second exposure. The author has verified this method using a specimen with
© 2011 Optical Society of America
OCIS Codes
(110.4190) Imaging systems : Multiple imaging
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
ToC Category:
Microscopy
History
Original Manuscript: March 28, 2011
Revised Manuscript: June 7, 2011
Manuscript Accepted: July 7, 2011
Published: August 29, 2011
Virtual Issues
Vol. 6, Iss. 10 Virtual Journal for Biomedical Optics
Citation
Koichiro Kishima, "Simple way of pinpointing the three-dimensional position of biomarkers in fluorescence microscopy using a through-focus exposure method," Appl. Opt. 50, 4989-4997 (2011)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-50-25-4989
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