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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 51, Iss. 21 — Jul. 20, 2012
  • pp: 5044–5050

Tomographic imaging of ratiometric fluorescence resonance energy transfer in scattering media

Yi Zhang, Xu Cao, Yanyan Xu, Qing Liu, Yue Zhang, Jianwen Luo, Xiaodong Liu, and Jing Bai  »View Author Affiliations

Applied Optics, Vol. 51, Issue 21, pp. 5044-5050 (2012)

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A method to visualize and quantify fluorescence resonance energy transfer (FRET) in scattering media is proposed. It combines the ratiometric FRET method with fluorescence molecular tomography (FMT) in continuous wave (CW) mode. To evaluate the performance of the proposed method, experiments on a tissue-mimicking phantom are carried out. The results demonstrate that the proposed approach is capable of visualizing and quantifying the FRET distribution in scattering media, which implies the further application of the ratiometric assay in in vivo studies.

© 2012 Optical Society of America

OCIS Codes
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6960) Medical optics and biotechnology : Tomography

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: January 3, 2012
Revised Manuscript: May 4, 2012
Manuscript Accepted: June 4, 2012
Published: July 11, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Yi Zhang, Xu Cao, Yanyan Xu, Qing Liu, Yue Zhang, Jianwen Luo, Xiaodong Liu, and Jing Bai, "Tomographic imaging of ratiometric fluorescence resonance energy transfer in scattering media," Appl. Opt. 51, 5044-5050 (2012)

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