Holographic optical coherence imaging is an en face form of optical coherence tomography that uses low-coherence digital holography as a coherence gate to select light from a chosen depth inside scattering tissue. By acquiring successive holograms at a high camera frame rate at a fixed depth, dynamic speckle provides information concerning dynamic light scattering from intracellular motility. Motility contrast imaging (MCI) uses living motion as a label-free and functional biomarker. MCI provides a new form of viability assay and also is applicable for proliferation and cytotoxicity assays. The results presented here demonstrate that low-coherence digital holography can extract viability information from biologically relevant three-dimensional (3D) tissue based on multicellular tumor spheroids by moving beyond the format of two-dimensional cell culture used for conventional high-content analysis. This paper also demonstrates the use of MCI for chemosensitivity assays on tumor exgrafts of excised ovarian cancer tumors responding to standard-of-care cisplatin chemotherapy. This ex vivo application extends the applicability of MCI beyond 3D tissue culture grown in vitro.
© 2012 Optical Society of America
Original Manuscript: August 21, 2012
Revised Manuscript: October 20, 2012
Manuscript Accepted: October 21, 2012
Published: November 28, 2012
Vol. 8, Iss. 2 Virtual Journal for Biomedical Optics
Ran An, John Turek, Daniela Elena Matei, and David Nolte, "Live tissue viability and chemosensitivity assays using digital holographic motility contrast imaging," Appl. Opt. 52, A300-A309 (2013)