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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 52, Iss. 30 — Oct. 20, 2013
  • pp: 7265–7270

Immersed transparent microsphere magnifying sub-diffraction-limited objects

Seoungjun Lee, Lin Li, Zengbo Wang, Wei Guo, Yinzhou Yan, and Tao Wang  »View Author Affiliations

Applied Optics, Vol. 52, Issue 30, pp. 7265-7270 (2013)

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The resolution of an optical microscope is restricted by the diffraction limit, which is approximately 200 nm for a white light source. We report that sub-diffraction-limited objects can be resolved in immersion liquids using a microsphere optical nanoscopy (MONS) technique. Image magnifications and resolutions were obtained experimentally and compared in different immersion liquids. We show that a 100 μm diameter barium titanate (BaTiO3) glass microsphere combined with a standard optical microscope can image sub-diffraction-limited objects with halogen light in three different media: water, 40% sugar solution, and microscope immersion oil. In this paper, the super-resolution imaging performance has been described with the three immersion liquid types and the mechanisms are discussed with Mie theory calculation in the field of a Poynting vector.

© 2013 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(110.2970) Imaging systems : Image detection systems
(180.0180) Microscopy : Microscopy

ToC Category:

Original Manuscript: May 28, 2013
Manuscript Accepted: September 12, 2013
Published: October 15, 2013

Seoungjun Lee, Lin Li, Zengbo Wang, Wei Guo, Yinzhou Yan, and Tao Wang, "Immersed transparent microsphere magnifying sub-diffraction-limited objects," Appl. Opt. 52, 7265-7270 (2013)

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