The identification and quantification of specific molecules are crucial for studying the pathophysiology of cells, tissues, and organs as well as diagnosis and treatment of diseases. Recent advances in holographic microspectroscopy, based on quantitative phase imaging or optical coherence tomography techniques, show promise for label-free noninvasive optical detection and quantification of specific molecules in living cells and tissues (e.g., hemoglobin protein). To provide important insight into the potential employment of holographic spectroscopy techniques in biological research and for related practical applications, we review the principles of holographic microspectroscopy techniques and highlight recent studies.
© 2014 Optical Society of America
Original Manuscript: April 30, 2014
Revised Manuscript: June 23, 2014
Manuscript Accepted: June 24, 2014
Published: August 1, 2014
JaeHwang Jung, Kyoohyun Kim, HyeonSeung Yu, KyeoReh Lee, SeoEun Lee, SeungHoon Nahm, HyunJoo Park, and YongKeun Park, "Biomedical applications of holographic microspectroscopy [Invited]," Appl. Opt. 53, G111-G122 (2014)