The influence of spherical aberration on axial imaging of confocal reflection microscopy is investigated. In particular, the effects of lens aperture size and of the first three orders of spherical aberration are inspected. It is shown both theoretically and experimentally that the aberrated axial response can be improved by slightly reducing the lens aperture size. The experimental results concerning the effect of the tube length on the axial response and the aberration compensation are also given.
© 1994 Optical Society of America
Original Manuscript: May 11, 1993
Revised Manuscript: August 3, 1993
Published: February 1, 1994
Colin J. R. Sheppard, Min Gu, Keith Brain, and Hao Zhou, "Influence of spherical aberration on axial imaging of confocal reflection microscopy," Appl. Opt. 33, 616-624 (1994)