An instrument which is suitable for investigating the light scattering properties of macromolecules in solution in the angular range 20° to 160° is described. A null modulation method is used to measure the ratio of the intensity I θ of the light scattered at an angle θ to the direction of the incident light to that (I θ ) incident on the scattering volume. This is accomplished by attenuating I0 until it equals I θ , this condition being found by an electronic detector. The value of I θ is known from a calibration experiment. Details are given of the optical arrangement, the detector, and the calibration and method of use of the apparatus. Adequate sensitivity, accuracy, and stability are demonstrated.
© 1962 Optical Society of America
Original Manuscript: November 28, 1961
Published: May 1, 1962
H. G. Jerrard and D. B. Sellen, "A New Apparatus for Light Scattering Studies," Appl. Opt. 1, 243-247 (1962)