Abstract

An instrument which is suitable for investigating the light scattering properties of macromolecules in solution in the angular range 20° to 160° is described. A null modulation method is used to measure the ratio of the intensity I_θ of the light scattered at an angle θ to the direction of the incident light to that (I_θ) incident on the scattering volume. This is accomplished by attenuating I₀ until it equals I_θ, this condition being found by an electronic detector. The value of I_θ is known from a calibration experiment. Details are given of the optical arrangement, the detector, and the calibration and method of use of the apparatus. Adequate sensitivity, accuracy, and stability are demonstrated.

© 1962 Optical Society of America

Automatic Photometer for Measuring the Angular Dissymmetry of Light Scattering*

F. J. Baum and F. W. Billmeyer
J. Opt. Soc. Am. 51(4) 452-456 (1961)

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Wilbur Kaye and A. J. Havlik
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Influence of Scattering on Fluorescence Spectra of Dilute Solutions Obtained with the Aminco–Bowman Spectrophotofluorometer

J. M. Price, M. Kaihara, and H. K. Howerton
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The Elimination of Scattered Light in Solar Spectrographs

J. W. Evans and J. Waddell
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Photoelectric Analysis of Polarized Light

Wolfgang Budde
Appl. Opt. 1(3) 201-205 (1962)