A holomicrographic system capable of recording the dynamic microscopic events occurring in a living tissue has been developed. This paper discusses the system parameters, the basic configuration, and initial test results. The reconstructed real image from the hologram can be studied with a conventional microscope. The holographic system uses a collecting lens both in recording and in reconstructing. Test results reported include the dissolving of a gas bubble under increased pressure and the movement of polystyrene balls when ejected from a needle. Present resolution is greater than 3 μ, and object velocities greater than 25 mm sec−1 can be recorded.
© 1971 Optical Society of America
Original Manuscript: April 3, 1970
Published: January 1, 1971
M. E. Cox, R. G. Buckles, and D. Whitlow, "Cineholomicroscopy of Small Animal Microcirculation," Appl. Opt. 10, 128-131 (1971)