A holomicrographic system capable of recording the dynamic microscopic events occurring in a living tissue has been developed. This paper discusses the system parameters, the basic configuration, and initial test results. The reconstructed real image from the hologram can be studied with a conventional microscope. The holographic system uses a collecting lens both in recording and in reconstructing. Test results reported include the dissolving of a gas bubble under increased pressure and the movement of polystyrene balls when ejected from a needle. Present resolution is greater than 3 µ, and object velocities greater than 25 mm sec-1 can be recorded.
M. E. Cox, R. G. Buckles, and D. Whitlow, "Cineholomicroscopy of Small Animal Microcirculation," Appl. Opt. 10, 128-131 (1971)