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Applied Optics

Applied Optics


  • Vol. 17, Iss. 6 — Mar. 15, 1978
  • pp: 944–950

Holographic microscopy

R. A. Briones, L. O. Heflinger, and R. F. Wuerker  »View Author Affiliations

Applied Optics, Vol. 17, Issue 6, pp. 944-950 (1978)

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An off-axis transmission holographic scheme, in which a 1:1 lens and a hologram are treated as a single rigid entity, is found to reconstruct a 3-D diffraction-limited image when reconstructed, with a reference beam reversed back through the original lens–hologram unit. Reconstruction can be performed with wavelengths other than the recording wavelength, provided achromatic lenses are used, and the reference beam angle is properly changed for reconstruction. Comparisons are made between He–Ne and ruby laser holograms. Two-micron resolution of the combustion of solid rocket propellants at high pressures is achieved at a working distance of 6 cm.

© 1978 Optical Society of America

Original Manuscript: July 25, 1977
Published: March 15, 1978

R. A. Briones, L. O. Heflinger, and R. F. Wuerker, "Holographic microscopy," Appl. Opt. 17, 944-950 (1978)

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  1. L. O. Heflinger et al., Appl. Opt. 17, 951 (1978). [CrossRef] [PubMed]
  2. H. Kogelnik, Bell Syst. Tech. J. 44, 2451 (1965).
  3. The 1951 resolution chart consists of both vertical and horizontal three-bar arrays whose spatial frequency (SF) varies according to the equationSF=2{column#+row#−16}(lp/mm).The smallest divisions correspond to the seventh column and six row, a spatial frequency of 228 lp/mm (spatial period of 4.4 μm or individual bar widths of 2.2 μm).
  4. Reconstruction of the 1951 chart holograms to highest resolutions could be achieved without recourse to the special alignment technique described in Ref. 2. For example, holograms recorded with a ruby laser and reconstructed with a He–Ne laser required a 9½° decrease in reference beam angle to compensate for the wavelength difference. The optimum reference beam angle was found by carefully tuning the beam relative to the hologram while microscopically observing the reconstructed image.
  5. A similar test was conducted with transparent 2.4 μm polystyrene spheres. The spheres were harder to reconstruct due to their lower contrast. They could just be seen in the reconstruction.
  6. Larger focusing lenses would permit recording even larger volumes at similar resolutions.
  7. These holograms were reconstructed back through a window section and a piece of glass that simulated the interference filter.

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