The construction and preliminary application of a thin-layer optical cell suitable for measuring absorption and fluorescence of highly absorbent solutions are described. The light path length is easily variable down to 2.5 µm with a commercial torque driver. Both absorption and fluorescence are measurable in a cell by conventional spectrophotometers and spectrofluorophotometers. The emission spectra of concentrated rhodamine B aqueous solutions up to 15 mM were measured with the thin-layer cell. The thin-layer cell showed great improvement in concentration quenching because of its very short light path length.
© 1984 Optical Society of America
Reiji Aoshima, Tsukasa Chiba, and Hiroshi Asai, "Thin-layer optical cell with light path length shorter than 10 µm for absorption and fluorescence measurement of highly absorbent solutions," Appl. Opt. 23, 597- 600 (1984)