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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 23, Iss. 4 — Feb. 15, 1984
  • pp: 597–600

Thin-layer optical cell with light path length shorter than 10 μm for absorption and fluorescence measurement of highly absorbent solutions

Reiji Aoshima, Tsukasa Chiba, and Hiroshi Asai  »View Author Affiliations


Applied Optics, Vol. 23, Issue 4, pp. 597-600 (1984)
http://dx.doi.org/10.1364/AO.23.000597


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Abstract

The construction and preliminary application of a thin-layer optical cell suitable for measuring absorption and fluorescence of highly absorbent solutions are described. The light path length is easily variable down to 2.5 μm with a commercial torque driver. Both absorption and fluorescence are measurable in a cell by conventional spectrophotometers and spectrofluorophotometers. The emission spectra of concentrated rhodamine B aqueous solutions up to 15 mM were measured with the thin-layer cell. The thin-layer cell showed great improvement in concentration quenching because of its very short light path length.

© 1984 Optical Society of America

History
Original Manuscript: September 28, 1983
Published: February 15, 1984

Citation
Reiji Aoshima, Tsukasa Chiba, and Hiroshi Asai, "Thin-layer optical cell with light path length shorter than 10 μm for absorption and fluorescence measurement of highly absorbent solutions," Appl. Opt. 23, 597-600 (1984)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-23-4-597


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References

  1. C. W. Robertson, D. Williams, J. Opt. Soc. Am. 61, 1316 (1971). [CrossRef]
  2. I. L. Tyler, G. Taylor, M. R. Querry, Appl. Opt. 17, 960 (1978). [CrossRef] [PubMed]
  3. G. Weber, F. W. J. Teale, Trans. Faraday Soc. 53, 640 (1958). [CrossRef]
  4. K. Horiuchi, H. Asai, Biochem. Biophys. Res. Commun. 97, 811 (1980). [CrossRef] [PubMed]

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