OSA's Digital Library

Applied Optics

Applied Optics


  • Vol. 23, Iss. 4 — Feb. 15, 1984
  • pp: 597–600

Thin-layer optical cell with light path length shorter than 10 μm for absorption and fluorescence measurement of highly absorbent solutions

Reiji Aoshima, Tsukasa Chiba, and Hiroshi Asai  »View Author Affiliations

Applied Optics, Vol. 23, Issue 4, pp. 597-600 (1984)

View Full Text Article

Enhanced HTML    Acrobat PDF (491 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



The construction and preliminary application of a thin-layer optical cell suitable for measuring absorption and fluorescence of highly absorbent solutions are described. The light path length is easily variable down to 2.5 μm with a commercial torque driver. Both absorption and fluorescence are measurable in a cell by conventional spectrophotometers and spectrofluorophotometers. The emission spectra of concentrated rhodamine B aqueous solutions up to 15 mM were measured with the thin-layer cell. The thin-layer cell showed great improvement in concentration quenching because of its very short light path length.

© 1984 Optical Society of America

Original Manuscript: September 28, 1983
Published: February 15, 1984

Reiji Aoshima, Tsukasa Chiba, and Hiroshi Asai, "Thin-layer optical cell with light path length shorter than 10 μm for absorption and fluorescence measurement of highly absorbent solutions," Appl. Opt. 23, 597-600 (1984)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. C. W. Robertson, D. Williams, J. Opt. Soc. Am. 61, 1316 (1971). [CrossRef]
  2. I. L. Tyler, G. Taylor, M. R. Querry, Appl. Opt. 17, 960 (1978). [CrossRef] [PubMed]
  3. G. Weber, F. W. J. Teale, Trans. Faraday Soc. 53, 640 (1958). [CrossRef]
  4. K. Horiuchi, H. Asai, Biochem. Biophys. Res. Commun. 97, 811 (1980). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3
Fig. 4 Fig. 5

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited